A molecular approach for the diagnosis of wood rotting disease in desert citrus

Manolis C. Demetriou, Gary A. Thompson, Glenn C Wright, Kathryn C. Taylor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Brown heartwood rot is an increasingly important problem in southwestern deserts, affecting numerous native tree species, and commercial lemon production. Two fungal pathogens, Coniophora eremophila and Antrodia sinuosa, have been identified as causal agents of brown heartwood rot in citrus. Development of effective disease management strategies relies on the ability to readily identify and distinguish between the pathogens; therefore we have developed molecular markers as an alternative to morphometric methods to differentiate these two economically important pathogens. Random amplified polymorphic DNA analysis identified four primers that revealed fungal polymorphisms. Polymorphic DNA fragments were cloned, sequenced, and used to create a 700 bp sequence characterized amplified region (SCAR) in C. eremophila isolates using sequence-specific primers, Conio7 and Conio8. Three fragments (300, 650, and 1000 bp) were amplified with Conio7 and Conio8 in A. sinuosa isolates. A rapid, small scale method for DNA extraction from fungus was developed to expedite the technique. With this method, the usefulness of the Conio7 and Conio8 was verified using new Coniophora eremophila and Antrodia sinuosa isolates from citrus.

Original languageEnglish (US)
Pages (from-to)1214-1219
Number of pages6
JournalMycologia
Volume92
Issue number1-6
StatePublished - Nov 2000

Fingerprint

Citrus
Antrodia
Coniophora
deserts
pathogen
desert
DNA
heartwood
pathogens
Disease Management
random amplified polymorphic DNA technique
polymorphism
lemons
Fungi
methodology
fungus
disease control
genetic polymorphism
fungi
genetic markers

Keywords

  • Antrodia sinuosa
  • Brown heartwood rot
  • Coniophora eremophila

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Demetriou, M. C., Thompson, G. A., Wright, G. C., & Taylor, K. C. (2000). A molecular approach for the diagnosis of wood rotting disease in desert citrus. Mycologia, 92(1-6), 1214-1219.

A molecular approach for the diagnosis of wood rotting disease in desert citrus. / Demetriou, Manolis C.; Thompson, Gary A.; Wright, Glenn C; Taylor, Kathryn C.

In: Mycologia, Vol. 92, No. 1-6, 11.2000, p. 1214-1219.

Research output: Contribution to journalArticle

Demetriou, MC, Thompson, GA, Wright, GC & Taylor, KC 2000, 'A molecular approach for the diagnosis of wood rotting disease in desert citrus', Mycologia, vol. 92, no. 1-6, pp. 1214-1219.
Demetriou MC, Thompson GA, Wright GC, Taylor KC. A molecular approach for the diagnosis of wood rotting disease in desert citrus. Mycologia. 2000 Nov;92(1-6):1214-1219.
Demetriou, Manolis C. ; Thompson, Gary A. ; Wright, Glenn C ; Taylor, Kathryn C. / A molecular approach for the diagnosis of wood rotting disease in desert citrus. In: Mycologia. 2000 ; Vol. 92, No. 1-6. pp. 1214-1219.
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