A molecular-based magnet test for malaria

R. M. Nalbandian, D. W. Sammons, M. Manley, L. Xie, Charles R Sterling, N. B. Egen, B. A. Gingras

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The molecular-based magnet test for malaria is shown to be more sensitive than the thin blood film test. The globally used thin blood film test is less sensitive because it uses preparation steps that result in the reduction of the absolute number of diagnostically pertinent erythrocytes. Several reports of diagnostic error with the thin film test and the thick film test have appeared in the literature. In marked contrast to the commonly accepted tests, the magnet test concurrently partitions and concentrates the infected erythrocytes present in the initial sample. The magnetic test permits a brief and sensitive microscopic-based enumeration of the malaria-infected erythrocytes in the enriched sample. Diagnostically pertinent hemozoin is simply identified through two of its specific molecular properties: paramagnetism and birefringence. The former property mediates the capture and enrichment of malaria-infected erythrocytes within the magnetic flux and the latter property manifests the characteristic birefringence demonstrated by polarized light.

Original languageEnglish (US)
Pages (from-to)57-64
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume103
Issue number1
StatePublished - 1995

Fingerprint

Magnets
Malaria
Erythrocytes
Birefringence
Hematologic Tests
Diagnostic Errors
Light

Keywords

  • Birefringence
  • Hemozoin
  • Magnetic separator
  • Malaria test
  • Plasmodium falciparum
  • Polarized light

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Nalbandian, R. M., Sammons, D. W., Manley, M., Xie, L., Sterling, C. R., Egen, N. B., & Gingras, B. A. (1995). A molecular-based magnet test for malaria. American Journal of Clinical Pathology, 103(1), 57-64.

A molecular-based magnet test for malaria. / Nalbandian, R. M.; Sammons, D. W.; Manley, M.; Xie, L.; Sterling, Charles R; Egen, N. B.; Gingras, B. A.

In: American Journal of Clinical Pathology, Vol. 103, No. 1, 1995, p. 57-64.

Research output: Contribution to journalArticle

Nalbandian, RM, Sammons, DW, Manley, M, Xie, L, Sterling, CR, Egen, NB & Gingras, BA 1995, 'A molecular-based magnet test for malaria', American Journal of Clinical Pathology, vol. 103, no. 1, pp. 57-64.
Nalbandian RM, Sammons DW, Manley M, Xie L, Sterling CR, Egen NB et al. A molecular-based magnet test for malaria. American Journal of Clinical Pathology. 1995;103(1):57-64.
Nalbandian, R. M. ; Sammons, D. W. ; Manley, M. ; Xie, L. ; Sterling, Charles R ; Egen, N. B. ; Gingras, B. A. / A molecular-based magnet test for malaria. In: American Journal of Clinical Pathology. 1995 ; Vol. 103, No. 1. pp. 57-64.
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