A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

A. Kleinhofs, A. Kilian, M. A. Saghai Maroof, R. M. Biyashev, P. Hayes, F. Q. Chen, N. Lapitan, A. Fenwick, T. K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, David A Kudrna, J. Bollinger, S. J. Knapp, B. Liu, M. Sorrells, M. Heun, J. D. Franckowiak, D. HoffmanR. Skadsen, B. J. Steffenson

Research output: Contribution to journalArticle

567 Scopus citations

Abstract

A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.

Original languageEnglish (US)
Pages (from-to)705-712
Number of pages8
JournalTheoretical And Applied Genetics
Volume86
Issue number6
DOIs
Publication statusPublished - Jul 1993
Externally publishedYes

    Fingerprint

Keywords

  • Barley
  • Centromeres
  • Genome
  • Mapping
  • RFLP

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Kleinhofs, A., Kilian, A., Saghai Maroof, M. A., Biyashev, R. M., Hayes, P., Chen, F. Q., ... Steffenson, B. J. (1993). A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theoretical And Applied Genetics, 86(6), 705-712. https://doi.org/10.1007/BF00222660