A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

A. Kleinhofs, A. Kilian, M. A. Saghai Maroof, R. M. Biyashev, P. Hayes, F. Q. Chen, N. Lapitan, A. Fenwick, T. K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, David A Kudrna, J. Bollinger, S. J. Knapp, B. Liu, M. Sorrells, M. Heun, J. D. Franckowiak, D. Hoffman & 2 others R. Skadsen, B. J. Steffenson

Research output: Contribution to journalArticle

561 Citations (Scopus)

Abstract

A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.

Original languageEnglish (US)
Pages (from-to)705-712
Number of pages8
JournalTheoretical And Applied Genetics
Volume86
Issue number6
DOIs
StatePublished - Jul 1993
Externally publishedYes

Fingerprint

Hordeum
Restriction Fragment Length Polymorphisms
Hordeum vulgare
Isoenzymes
isozymes
barley
Genome
chromosomes
genome
Chromosomes
restriction fragment length polymorphism
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Disease Resistance
Chromosomes, Human, Pair 2
Centromere
Chromosomes, Human, Pair 1
DNA
loci

Keywords

  • Barley
  • Centromeres
  • Genome
  • Mapping
  • RFLP

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Kleinhofs, A., Kilian, A., Saghai Maroof, M. A., Biyashev, R. M., Hayes, P., Chen, F. Q., ... Steffenson, B. J. (1993). A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theoretical And Applied Genetics, 86(6), 705-712. https://doi.org/10.1007/BF00222660

A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. / Kleinhofs, A.; Kilian, A.; Saghai Maroof, M. A.; Biyashev, R. M.; Hayes, P.; Chen, F. Q.; Lapitan, N.; Fenwick, A.; Blake, T. K.; Kanazin, V.; Ananiev, E.; Dahleen, L.; Kudrna, David A; Bollinger, J.; Knapp, S. J.; Liu, B.; Sorrells, M.; Heun, M.; Franckowiak, J. D.; Hoffman, D.; Skadsen, R.; Steffenson, B. J.

In: Theoretical And Applied Genetics, Vol. 86, No. 6, 07.1993, p. 705-712.

Research output: Contribution to journalArticle

Kleinhofs, A, Kilian, A, Saghai Maroof, MA, Biyashev, RM, Hayes, P, Chen, FQ, Lapitan, N, Fenwick, A, Blake, TK, Kanazin, V, Ananiev, E, Dahleen, L, Kudrna, DA, Bollinger, J, Knapp, SJ, Liu, B, Sorrells, M, Heun, M, Franckowiak, JD, Hoffman, D, Skadsen, R & Steffenson, BJ 1993, 'A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome', Theoretical And Applied Genetics, vol. 86, no. 6, pp. 705-712. https://doi.org/10.1007/BF00222660
Kleinhofs A, Kilian A, Saghai Maroof MA, Biyashev RM, Hayes P, Chen FQ et al. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theoretical And Applied Genetics. 1993 Jul;86(6):705-712. https://doi.org/10.1007/BF00222660
Kleinhofs, A. ; Kilian, A. ; Saghai Maroof, M. A. ; Biyashev, R. M. ; Hayes, P. ; Chen, F. Q. ; Lapitan, N. ; Fenwick, A. ; Blake, T. K. ; Kanazin, V. ; Ananiev, E. ; Dahleen, L. ; Kudrna, David A ; Bollinger, J. ; Knapp, S. J. ; Liu, B. ; Sorrells, M. ; Heun, M. ; Franckowiak, J. D. ; Hoffman, D. ; Skadsen, R. ; Steffenson, B. J. / A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. In: Theoretical And Applied Genetics. 1993 ; Vol. 86, No. 6. pp. 705-712.
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