A mouse model of permanent focal ischemia

Distal middle cerebral artery occlusion

Kristian Doyle, Marion S. Buckwalter

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Here we provide a standardized protocol for performing distal middle cerebral artery occlusion (DMCAO) in mice. DMCAO is a method of inducing permanent focal ischemia that is commonly used as a rodent stroke model. To perform DMCAO a temporal craniotomy is performed, and the middle cerebral artery (MCA) is permanently ligated at a point downstream of the lenticulostriate branches. The size of the lesion produced by this surgery is strain dependent. In C57BL/6J mice, DMCAO produces an infarct predominantly restricted to the barrel region of the somatosensory cortex, but in BALB/cJ mice, DMCAO generates a much larger lesion that incorporates more of the somatosensory cortex and part of the M1 region of the motor cortex. The larger lesion produced by DMCAO in BALB/cJ mice produces a clearer sensorimotor deficit, which is useful for investigating recovery from stroke. We also describe how to modify DMCAO in C57BL/6J mice with the application of hypoxia to generate a lesion and sensorimotor deficit that are similar in size to those produced by DMCAO alone in BALB/cJ mice. This is extremely useful for stroke experiments that require a robust sensorimotor deficit in transgenic mice created on a C57BL/6J background.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages103-110
Number of pages8
Volume1135
ISBN (Print)9781493903191
DOIs
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1135
ISSN (Print)10643745

Fingerprint

Middle Cerebral Artery Infarction
Ischemia
Somatosensory Cortex
Stroke
Inbred C57BL Mouse
Craniotomy
Middle Cerebral Artery
Motor Cortex
Transgenic Mice
Rodentia

Keywords

  • DH stroke
  • Distal middle cerebral artery occlusion
  • Mouse stroke model
  • Permanent focal ischemia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Doyle, K., & Buckwalter, M. S. (2014). A mouse model of permanent focal ischemia: Distal middle cerebral artery occlusion. In Methods in Molecular Biology (Vol. 1135, pp. 103-110). (Methods in Molecular Biology; Vol. 1135). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-320-7_9

A mouse model of permanent focal ischemia : Distal middle cerebral artery occlusion. / Doyle, Kristian; Buckwalter, Marion S.

Methods in Molecular Biology. Vol. 1135 Humana Press Inc., 2014. p. 103-110 (Methods in Molecular Biology; Vol. 1135).

Research output: Chapter in Book/Report/Conference proceedingChapter

Doyle, K & Buckwalter, MS 2014, A mouse model of permanent focal ischemia: Distal middle cerebral artery occlusion. in Methods in Molecular Biology. vol. 1135, Methods in Molecular Biology, vol. 1135, Humana Press Inc., pp. 103-110. https://doi.org/10.1007/978-1-4939-320-7_9
Doyle K, Buckwalter MS. A mouse model of permanent focal ischemia: Distal middle cerebral artery occlusion. In Methods in Molecular Biology. Vol. 1135. Humana Press Inc. 2014. p. 103-110. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-320-7_9
Doyle, Kristian ; Buckwalter, Marion S. / A mouse model of permanent focal ischemia : Distal middle cerebral artery occlusion. Methods in Molecular Biology. Vol. 1135 Humana Press Inc., 2014. pp. 103-110 (Methods in Molecular Biology).
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