A mouse model of permanent focal ischemia: Distal middle cerebral artery occlusion

Kristian Doyle, Marion S. Buckwalter

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Scopus citations

Abstract

Here we provide a standardized protocol for performing distal middle cerebral artery occlusion (DMCAO) in mice. DMCAO is a method of inducing permanent focal ischemia that is commonly used as a rodent stroke model. To perform DMCAO a temporal craniotomy is performed, and the middle cerebral artery (MCA) is permanently ligated at a point downstream of the lenticulostriate branches. The size of the lesion produced by this surgery is strain dependent. In C57BL/6J mice, DMCAO produces an infarct predominantly restricted to the barrel region of the somatosensory cortex, but in BALB/cJ mice, DMCAO generates a much larger lesion that incorporates more of the somatosensory cortex and part of the M1 region of the motor cortex. The larger lesion produced by DMCAO in BALB/cJ mice produces a clearer sensorimotor deficit, which is useful for investigating recovery from stroke. We also describe how to modify DMCAO in C57BL/6J mice with the application of hypoxia to generate a lesion and sensorimotor deficit that are similar in size to those produced by DMCAO alone in BALB/cJ mice. This is extremely useful for stroke experiments that require a robust sensorimotor deficit in transgenic mice created on a C57BL/6J background.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages103-110
Number of pages8
Volume1135
ISBN (Print)9781493903191
DOIs
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1135
ISSN (Print)10643745

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Keywords

  • DH stroke
  • Distal middle cerebral artery occlusion
  • Mouse stroke model
  • Permanent focal ischemia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Doyle, K., & Buckwalter, M. S. (2014). A mouse model of permanent focal ischemia: Distal middle cerebral artery occlusion. In Methods in Molecular Biology (Vol. 1135, pp. 103-110). (Methods in Molecular Biology; Vol. 1135). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-320-7_9