Abstract
A multimodal gold nanorod-locked nucleic acid (GNR-LNA) approach is developed for perturbing and monitoring the spatiotemporal gene expression dynamics at the single cell level. We demonstrated (1) LNA-based detection of mRNA expression in single living cells as well as lung and liver tissues, (2) plasmonic GNR-mediated photothermal ablation of mice lung tissues for perturbing gene expression and single cell ablation, and (3) monitoring the spatiotemporal gene expression dynamics of individual cells after photothermal ablation. Experimental results demonstrated the applicability and specificity of GNR-LNA probe for detecting individual cell mRNA expression, providing an effective approach for investigating complex tissue morphogenic and regeneration processes.
| Original language | English (US) |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1116-1118 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806476 |
| State | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country | United States |
| City | San Antonio |
| Period | 10/26/14 → 10/30/14 |
Keywords
- GNR-LNA
- MRNA expression
- Photothermal ablation
- Tissue morphogenesis
ASJC Scopus subject areas
- Control and Systems Engineering