A multimodal gold nanorod-locked nucleic acid approach for probing individual cell gene expressions in living tissues

S. Wang, R. Riahi, M. Long, Donna Zhang, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A multimodal gold nanorod-locked nucleic acid (GNR-LNA) approach is developed for perturbing and monitoring the spatiotemporal gene expression dynamics at the single cell level. We demonstrated (1) LNA-based detection of mRNA expression in single living cells as well as lung and liver tissues, (2) plasmonic GNR-mediated photothermal ablation of mice lung tissues for perturbing gene expression and single cell ablation, and (3) monitoring the spatiotemporal gene expression dynamics of individual cells after photothermal ablation. Experimental results demonstrated the applicability and specificity of GNR-LNA probe for detecting individual cell mRNA expression, providing an effective approach for investigating complex tissue morphogenic and regeneration processes.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1116-1118
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • GNR-LNA
  • MRNA expression
  • Photothermal ablation
  • Tissue morphogenesis

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Wang, S., Riahi, R., Long, M., Zhang, D., & Wong, P. K. (2014). A multimodal gold nanorod-locked nucleic acid approach for probing individual cell gene expressions in living tissues. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1116-1118). Chemical and Biological Microsystems Society.