A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis

Jian Gao, Lindsie Jeffries, Kathleen E. Mach, David W. Craft, Neal J. Thomas, Vincent Gau, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Accurate and timely detection of bacterial pathogens will improve the clinical management of infections. Herein, we demonstrate an electrochemical biosensor that directly detects bacteria in human blood samples, resulting in the rapid diagnosis of a bloodstream infection. The multiplex biosensor detects the species-specific sequences of the 16S ribosomal RNA of bacteria for pathogen identification in physiological samples without preamplification. The analytical performance characteristics of the biosensor, including the limit of detection and probe cross-reactivity, are evaluated systematically. The feasibility of the biosensor for a diagnosis of a bloodstream infection is demonstrated by identifying bacterial clinical isolates spiked in whole blood and blood culture samples that were tested positive for bacteria. The electrochemical biosensor correctly identifies all the species in the samples with 100% concordance to microbiological analysis.

Original languageEnglish (US)
Pages (from-to)466-474
Number of pages9
JournalSLAS Technology
Issue number4
StatePublished - Aug 1 2017


  • RNA
  • biosensor
  • blood
  • diagnostics
  • infection

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology


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