A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis

Jian Gao, Lindsie Jeffries, Kathleen E. Mach, David W. Craft, Neal J. Thomas, Vincent Gau, Joseph C. Liao, Pak Kin Wong

Research output: Research - peer-reviewArticle

  • 3 Citations

Abstract

Accurate and timely detection of bacterial pathogens will improve the clinical management of infections. Herein, we demonstrate an electrochemical biosensor that directly detects bacteria in human blood samples, resulting in the rapid diagnosis of a bloodstream infection. The multiplex biosensor detects the species-specific sequences of the 16S ribosomal RNA of bacteria for pathogen identification in physiological samples without preamplification. The analytical performance characteristics of the biosensor, including the limit of detection and probe cross-reactivity, are evaluated systematically. The feasibility of the biosensor for a diagnosis of a bloodstream infection is demonstrated by identifying bacterial clinical isolates spiked in whole blood and blood culture samples that were tested positive for bacteria. The electrochemical biosensor correctly identifies all the species in the samples with 100% concordance to microbiological analysis.

LanguageEnglish (US)
Pages466-474
Number of pages9
JournalSLAS Technology
Volume22
Issue number4
DOIs
StatePublished - Aug 1 2017
Externally publishedYes

Fingerprint

Biosensing Techniques
Infection
Biosensors
Bacteria
Blood
Pathogens
16S Ribosomal RNA
Limit of Detection
Blood Culture
Ribosomal RNA

Keywords

  • biosensor
  • blood
  • diagnostics
  • infection
  • RNA

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Computer Science Applications

Cite this

Gao, J., Jeffries, L., Mach, K. E., Craft, D. W., Thomas, N. J., Gau, V., ... Wong, P. K. (2017). A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis. SLAS Technology, 22(4), 466-474. DOI: 10.1177/2211068216651232

A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis. / Gao, Jian; Jeffries, Lindsie; Mach, Kathleen E.; Craft, David W.; Thomas, Neal J.; Gau, Vincent; Liao, Joseph C.; Wong, Pak Kin.

In: SLAS Technology, Vol. 22, No. 4, 01.08.2017, p. 466-474.

Research output: Research - peer-reviewArticle

Gao, J, Jeffries, L, Mach, KE, Craft, DW, Thomas, NJ, Gau, V, Liao, JC & Wong, PK 2017, 'A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis' SLAS Technology, vol 22, no. 4, pp. 466-474. DOI: 10.1177/2211068216651232
Gao J, Jeffries L, Mach KE, Craft DW, Thomas NJ, Gau V et al. A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis. SLAS Technology. 2017 Aug 1;22(4):466-474. Available from, DOI: 10.1177/2211068216651232
Gao, Jian ; Jeffries, Lindsie ; Mach, Kathleen E. ; Craft, David W. ; Thomas, Neal J. ; Gau, Vincent ; Liao, Joseph C. ; Wong, Pak Kin. / A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis. In: SLAS Technology. 2017 ; Vol. 22, No. 4. pp. 466-474
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