A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma

Meser M. Ali, Mohammed P I Bhuiyan, Branislava Janic, Nadimpalli R S Varma, Tom Mikkelsen, James R. Ewing, Robert A. Knight, Mark "Marty" Pagel, Ali S. Arbab

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Aim: The authors have investigated the usefulness of in vivo chemical exchange saturation transfer MRI for detecting gliomas using a dual-modality imaging contrast agent. Materials & methods: A paramagnetic chemical exchange saturation transfer MRI contrast agent, Eu-1,4,7,10- tetraazacclododecane-1,4,7,10-tetraacetic acid-Gly4 and a fluorescent agent, DyLight® 680, were conjugated to a generation 5 polyamidoamine dendrimer to create the dual-modality, nano-sized imaging contrast agent. Results: The agent was detected with in vivo chemical exchange saturation transfer MRI in an U87 glioma model. These results were validated using in vivo and ex vivo fluorescence imaging. Conclusion: This study demonstrated the merits of using a nano-sized imaging contrast agent for detecting gliomas and using a dual-modality agent for detecting gliomas at different spatial scales. Original submitted 9 January 2012; Revised submitted 18 April 2012; Published online 14 August 201.

Original languageEnglish (US)
Pages (from-to)1827-1837
Number of pages11
JournalNanomedicine
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 2012

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Keywords

  • brain tumor
  • contrast agent
  • dendrimer
  • MRI
  • pharmacokinetic

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Ali, M. M., Bhuiyan, M. P. I., Janic, B., Varma, N. R. S., Mikkelsen, T., Ewing, J. R., ... Arbab, A. S. (2012). A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma. Nanomedicine, 7(12), 1827-1837. https://doi.org/10.2217/nnm.12.92