A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta

Timothy G. Kingan, David B. Teplow, Jessica M. Phillips, John P. Riehm, K. Ranga Rao, John G Hildebrand, Uwe Homberg, Ann E. Kammer, Ian Jardine, Patrick R. Griffin, Donald F. Hunt

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

We have purified a FMRFamide-like peptide from extracts of brain-subesophageal ganglion of the moth, Manduca sexta. The purification was monitored with a new, competitive ELISA, and accomplished with ion exchange and reverse-phase HPLC. The peptide structure was determined by a combination of tandem mass spectrometry and automated Edman degradation. The amino acid sequence of the peptide is <Glu-Asp-Val-Val-His-Ser-Phe-Leu-Arg-Phe-amide (pEDVVHSFLRF-NH2). In a separate purification, an identical peptide was isolated from extracts of brain-associated neurohemal structures. We have named this peptide ManducaFLRFamide, to indicate its homology with other members of the "FMRFamide" family. In bioassays, chemically synthesized peptide increased the force of neurally evoked contractions in the major power-producing flight muscles, the dorsal longitudinal muscles. This observation suggests that hormonally released ManducaFLRFamide may play a role in sustaining or promoting the flight behavior necessary for mate-seeking (in males) or oviposition (in females) in sphingid moths.

Original languageEnglish (US)
Pages (from-to)849-856
Number of pages8
JournalPeptides
Volume11
Issue number4
DOIs
StatePublished - 1990

Fingerprint

FMRFamide
Manduca
Peptides
Moths
Purification
Muscle
Brain
Muscles
Oviposition
Bioassay
Ion Exchange
Tandem Mass Spectrometry
Ganglia
Biological Assay
Mass spectrometry
Amino Acid Sequence
Ion exchange
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Amino Acids

Keywords

  • FMRFamide family
  • Hawkmoth
  • HPLC
  • Ion exchange
  • Peptide purification

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Kingan, T. G., Teplow, D. B., Phillips, J. M., Riehm, J. P., Ranga Rao, K., Hildebrand, J. G., ... Hunt, D. F. (1990). A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta. Peptides, 11(4), 849-856. https://doi.org/10.1016/0196-9781(90)90203-H

A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta. / Kingan, Timothy G.; Teplow, David B.; Phillips, Jessica M.; Riehm, John P.; Ranga Rao, K.; Hildebrand, John G; Homberg, Uwe; Kammer, Ann E.; Jardine, Ian; Griffin, Patrick R.; Hunt, Donald F.

In: Peptides, Vol. 11, No. 4, 1990, p. 849-856.

Research output: Contribution to journalArticle

Kingan, TG, Teplow, DB, Phillips, JM, Riehm, JP, Ranga Rao, K, Hildebrand, JG, Homberg, U, Kammer, AE, Jardine, I, Griffin, PR & Hunt, DF 1990, 'A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta', Peptides, vol. 11, no. 4, pp. 849-856. https://doi.org/10.1016/0196-9781(90)90203-H
Kingan, Timothy G. ; Teplow, David B. ; Phillips, Jessica M. ; Riehm, John P. ; Ranga Rao, K. ; Hildebrand, John G ; Homberg, Uwe ; Kammer, Ann E. ; Jardine, Ian ; Griffin, Patrick R. ; Hunt, Donald F. / A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta. In: Peptides. 1990 ; Vol. 11, No. 4. pp. 849-856.
@article{19764d3c88d248178c884b2c9dfb0844,
title = "A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta",
abstract = "We have purified a FMRFamide-like peptide from extracts of brain-subesophageal ganglion of the moth, Manduca sexta. The purification was monitored with a new, competitive ELISA, and accomplished with ion exchange and reverse-phase HPLC. The peptide structure was determined by a combination of tandem mass spectrometry and automated Edman degradation. The amino acid sequence of the peptide is <Glu-Asp-Val-Val-His-Ser-Phe-Leu-Arg-Phe-amide (pEDVVHSFLRF-NH2). In a separate purification, an identical peptide was isolated from extracts of brain-associated neurohemal structures. We have named this peptide ManducaFLRFamide, to indicate its homology with other members of the {"}FMRFamide{"} family. In bioassays, chemically synthesized peptide increased the force of neurally evoked contractions in the major power-producing flight muscles, the dorsal longitudinal muscles. This observation suggests that hormonally released ManducaFLRFamide may play a role in sustaining or promoting the flight behavior necessary for mate-seeking (in males) or oviposition (in females) in sphingid moths.",
keywords = "FMRFamide family, Hawkmoth, HPLC, Ion exchange, Peptide purification",
author = "Kingan, {Timothy G.} and Teplow, {David B.} and Phillips, {Jessica M.} and Riehm, {John P.} and {Ranga Rao}, K. and Hildebrand, {John G} and Uwe Homberg and Kammer, {Ann E.} and Ian Jardine and Griffin, {Patrick R.} and Hunt, {Donald F.}",
year = "1990",
doi = "10.1016/0196-9781(90)90203-H",
language = "English (US)",
volume = "11",
pages = "849--856",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta

AU - Kingan, Timothy G.

AU - Teplow, David B.

AU - Phillips, Jessica M.

AU - Riehm, John P.

AU - Ranga Rao, K.

AU - Hildebrand, John G

AU - Homberg, Uwe

AU - Kammer, Ann E.

AU - Jardine, Ian

AU - Griffin, Patrick R.

AU - Hunt, Donald F.

PY - 1990

Y1 - 1990

N2 - We have purified a FMRFamide-like peptide from extracts of brain-subesophageal ganglion of the moth, Manduca sexta. The purification was monitored with a new, competitive ELISA, and accomplished with ion exchange and reverse-phase HPLC. The peptide structure was determined by a combination of tandem mass spectrometry and automated Edman degradation. The amino acid sequence of the peptide is <Glu-Asp-Val-Val-His-Ser-Phe-Leu-Arg-Phe-amide (pEDVVHSFLRF-NH2). In a separate purification, an identical peptide was isolated from extracts of brain-associated neurohemal structures. We have named this peptide ManducaFLRFamide, to indicate its homology with other members of the "FMRFamide" family. In bioassays, chemically synthesized peptide increased the force of neurally evoked contractions in the major power-producing flight muscles, the dorsal longitudinal muscles. This observation suggests that hormonally released ManducaFLRFamide may play a role in sustaining or promoting the flight behavior necessary for mate-seeking (in males) or oviposition (in females) in sphingid moths.

AB - We have purified a FMRFamide-like peptide from extracts of brain-subesophageal ganglion of the moth, Manduca sexta. The purification was monitored with a new, competitive ELISA, and accomplished with ion exchange and reverse-phase HPLC. The peptide structure was determined by a combination of tandem mass spectrometry and automated Edman degradation. The amino acid sequence of the peptide is <Glu-Asp-Val-Val-His-Ser-Phe-Leu-Arg-Phe-amide (pEDVVHSFLRF-NH2). In a separate purification, an identical peptide was isolated from extracts of brain-associated neurohemal structures. We have named this peptide ManducaFLRFamide, to indicate its homology with other members of the "FMRFamide" family. In bioassays, chemically synthesized peptide increased the force of neurally evoked contractions in the major power-producing flight muscles, the dorsal longitudinal muscles. This observation suggests that hormonally released ManducaFLRFamide may play a role in sustaining or promoting the flight behavior necessary for mate-seeking (in males) or oviposition (in females) in sphingid moths.

KW - FMRFamide family

KW - Hawkmoth

KW - HPLC

KW - Ion exchange

KW - Peptide purification

UR - http://www.scopus.com/inward/record.url?scp=0025078553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025078553&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(90)90203-H

DO - 10.1016/0196-9781(90)90203-H

M3 - Article

C2 - 2235684

AN - SCOPUS:0025078553

VL - 11

SP - 849

EP - 856

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 4

ER -