Abstract
The Standard-Based Polynomial Interpolation (SBPIn) method is a new simple three-step protocol proposed to address common gel-to-gel variations for the comparison of sample profiles across multiple DGGE gels. The advantages of this method include no requirement for additional software or modification of the standard DGGE protocol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 173-177 |
| Number of pages | 5 |
| Journal | Journal of Microbiological Methods |
| Volume | 92 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 5 2013 |
Keywords
- Community fingerprint
- DGGE
- Gel-to-gel variations
- Microbial ecology
- Molecular standard
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)
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Valentín-Vargas, A., Chorover, J., & Maier, R. M. (2013). A New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices. Journal of Microbiological Methods, 92(2), 173-177. https://doi.org/10.1016/j.mimet.2012.12.001