A New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices

Alexis Valentín-Vargas, Jon Chorover, Raina M. Maier

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The Standard-Based Polynomial Interpolation (SBPIn) method is a new simple three-step protocol proposed to address common gel-to-gel variations for the comparison of sample profiles across multiple DGGE gels. The advantages of this method include no requirement for additional software or modification of the standard DGGE protocol.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalJournal of Microbiological Methods
Volume92
Issue number2
DOIs
StatePublished - Feb 5 2013

Keywords

  • Community fingerprint
  • DGGE
  • Gel-to-gel variations
  • Microbial ecology
  • Molecular standard

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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