A novel concept for protein microarray: Land-contrast BioCD

Xuefeng Wang; Ming Zhao; David D. Nolte

doi:10.1117/12.809358

A novel concept for protein microarray: Land-contrast BioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Optical Sciences, College of

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We present a new type of protein microarray called the Land-contrast (LC) BioCD in which imaging contrast is induced by a patterned substrate rather than by patterned protein. This is realized by etching spot patterns in a silicon wafer. On the spot region the silicon dioxide thickness is 140 nm and on the land it is 77 nm. The spot and the land have equal reflectance but opposite interferometric quadrature responses for protein layer. Protein is evenly immobilized on the entire chip and detected by reflectometry. Therefore there is no need for protein printing, nor spectrometers, nor high angles nor polarization control to image the surface-bound protein. The LC BioCD can facilitate research on protein microarrays.

Original language	English (US)
Title of host publication	Design and Quality for Biomedical Technologies II
DOIs	https://doi.org/10.1117/12.809358
State	Published - Sep 18 2009
Event	Design and Quality for Biomedical Technologies II - San Jose, CA, United States Duration: Jan 26 2009 → Jan 26 2009

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7170
ISSN (Print)	1605-7422

Other

Other	Design and Quality for Biomedical Technologies II
Country	United States
City	San Jose, CA
Period	1/26/09 → 1/26/09

Keywords

BioCD
Label-free
Land contrast
Optical biosensor
Protein microarray

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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Wang, X., Zhao, M., & Nolte, D. D. (2009). A novel concept for protein microarray: Land-contrast BioCD. In Design and Quality for Biomedical Technologies II [717003] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7170). https://doi.org/10.1117/12.809358

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abstract = "We present a new type of protein microarray called the Land-contrast (LC) BioCD in which imaging contrast is induced by a patterned substrate rather than by patterned protein. This is realized by etching spot patterns in a silicon wafer. On the spot region the silicon dioxide thickness is 140 nm and on the land it is 77 nm. The spot and the land have equal reflectance but opposite interferometric quadrature responses for protein layer. Protein is evenly immobilized on the entire chip and detected by reflectometry. Therefore there is no need for protein printing, nor spectrometers, nor high angles nor polarization control to image the surface-bound protein. The LC BioCD can facilitate research on protein microarrays.",

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