A novel concept for protein microarray: Land-contrast BioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


We present a new type of protein microarray called the Land-contrast (LC) BioCD in which imaging contrast is induced by a patterned substrate rather than by patterned protein. This is realized by etching spot patterns in a silicon wafer. On the spot region the silicon dioxide thickness is 140 nm and on the land it is 77 nm. The spot and the land have equal reflectance but opposite interferometric quadrature responses for protein layer. Protein is evenly immobilized on the entire chip and detected by reflectometry. Therefore there is no need for protein printing, nor spectrometers, nor high angles nor polarization control to image the surface-bound protein. The LC BioCD can facilitate research on protein microarrays.

Original languageEnglish (US)
Title of host publicationDesign and Quality for Biomedical Technologies II
StatePublished - Sep 18 2009
EventDesign and Quality for Biomedical Technologies II - San Jose, CA, United States
Duration: Jan 26 2009Jan 26 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherDesign and Quality for Biomedical Technologies II
Country/TerritoryUnited States
CitySan Jose, CA


  • BioCD
  • Label-free
  • Land contrast
  • Optical biosensor
  • Protein microarray

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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