A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA

Danzhou Yang, S. S G E Van Boom, J. Reedijk, J. H. Van Boom, N. Farrell, A. H J Wang -

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The bifunctional platinum compound, [{trans- PtCl(NH3)2}2(H2N(CH2)4NH2)]2+, forms a stable adduct with the self- complementary DNA oligomer CATGCATG, with the two platinum atoms coordinated at the N7 positions of the two symmetrical G4 nucleotides. The NMR-derived structure shows that the DNA octamer forms a novel hairpin structure with the platinated G4 residue adopting a syn conformation and the guanine base in the minor groove. Two such hairpins stack end-over-end and are linked together by the butanediamine tether to form a dumbbell structure. Such unusual structural distortion is different from that of the anticancer drug cisplatin-DNA adduct and may provide clues to explain the distinct biological activities of the two compounds.

Original languageEnglish (US)
Pages (from-to)577-586
Number of pages10
JournalNature Structural Biology
Volume2
Issue number7
StatePublished - 1995
Externally publishedYes

Fingerprint

Platinum Compounds
Guanine
Bioactivity
Platinum
Oligomers
Conformations
Nucleotides
Complementary DNA
Nuclear magnetic resonance
Atoms
DNA
Pharmaceutical Preparations
cisplatin-DNA adduct

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Yang, D., Van Boom, S. S. G. E., Reedijk, J., Van Boom, J. H., Farrell, N., & Wang -, A. H. J. (1995). A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA. Nature Structural Biology, 2(7), 577-586.

A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA. / Yang, Danzhou; Van Boom, S. S G E; Reedijk, J.; Van Boom, J. H.; Farrell, N.; Wang -, A. H J.

In: Nature Structural Biology, Vol. 2, No. 7, 1995, p. 577-586.

Research output: Contribution to journalArticle

Yang, D, Van Boom, SSGE, Reedijk, J, Van Boom, JH, Farrell, N & Wang -, AHJ 1995, 'A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA', Nature Structural Biology, vol. 2, no. 7, pp. 577-586.
Yang, Danzhou ; Van Boom, S. S G E ; Reedijk, J. ; Van Boom, J. H. ; Farrell, N. ; Wang -, A. H J. / A novel DNA structure induced by the anticancer bisplatinum compound crosslinked to a GpC site in DNA. In: Nature Structural Biology. 1995 ; Vol. 2, No. 7. pp. 577-586.
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