A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

Jie Fan, Xinxin Ding, Yongping Jiang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34+ cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34+ cells, which is located in the first 104 amino acids from the NH2-terminus.

Original languageEnglish (US)
Article number73
JournalJournal of Hematology and Oncology
Volume5
DOIs
StatePublished - 2012
Externally publishedYes

Keywords

  • Hematopoietic stem cell
  • Monoclonal antibody
  • Stem cell factor
  • Umbilical cord blood

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Oncology
  • Cancer Research

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