A novel target cell antigen involved in the NK-like lytic activity of antigen-specific cytotoxic T lymphocytes

David T. Harris, Liliana Jaso-Friedmann, Donald L. Evans

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Monoclonal antibodies (mAbs) have been derived which identify a target cell antigen involved in human natural killer (NK) cell lysis. The effects of the mAbs on the NK-like cytotoxic activity exhibited by different populations of human cytolytic T cells were examined. The anti-target cell mAbs 1E7 and 18C2 inhibited the lysis of K562 target cells by endogenous NK cells, antigen-specific cytotoxic T lymphocytes (CTL) with NK-like activity, and non-major histocompatibility complex (MHC)-restricted T cells. Cytolysis of K562 target cells was not affected by treatment of the target cells with the anti-class I HLA mAb W6/32. Further, the anti-target cell mAbs had no effect on antigen-specific lysis by the CTL. The mAb W6/32, however, inhibited the antigen-specific killing. Experiments at the single-cell level revealed that the anti-target cell mAbs inhibited the formation of conjugates between the effector cells and K562 tumor cells but had no effect on CTL binding to the antigen-specific target cells. Thus, antigen-specific CTL exhibiting NK-like lytic function appeared to recognize a novel target cell antigen that is distinct from typical MHC antigens and is identical to the target-cell antigen recognized by true NK cells.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalImmunology Letters
Volume38
Issue number1
DOIs
StatePublished - Sep 1993

Keywords

  • Antigen recognition
  • Cytotoxic T lymphocyte
  • Natural killer cell
  • Target antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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