A novel type of adhering junction in an epithelioid tumorigenic rat cell culture line

Monika Schmelz, Dennis L. Way, Peter Borgs, Wiebke K. Peitsch, Hannelore Schmidt, Marlys H Witte, Charles L. Witte, Werner W. Franke, Roland Moll

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Two major types of plaque-bearing adhering junctions are commonly distinguished: the actin microfilament-anchoring adhaerens junctions (AJs) and the desmosomes anchoring intermediate-sized filaments (IFs). Both types of junction usually possess the common plaque protein, plakoglobin, whereas the other plaque proteins and the transmembrane cadherins are mutually exclusive. For example, AJs contain E-, N-, or P-cadherin in combination with α- and β-catenin, vinculin and α-actinin, whereas in desmosomes, desmogleins and desmocollins are associated with desmoplakin and one or several of the plakophilins (PP1-3). Here we describe a novel type of adhering junction comprising proteins of both AJs and desmosomes and the tight junction (TJ) plaque protein, ZO-1, in a newly established, liver-derived tumorigenic rat cell line (RMEC-1). By immunofluorescence microscopy, cell-cell contacts are characterized by mostly continuous-appearing lines which are usually resolved by electron microscopy as extended arrays of closely spaced small plaque subunits. These plaque-covered regions are positive for plakoglobin, α- and β-catenin, the arm-repeat protein p120, vinculin, desmoplakin and protein ZO-1. They are positive for E-cadherin in cultures early on in passaging, but tend to turn negative for all known cadherins in densely grown cultures. On immunoblotting SDS-PAGE-separated proteins from dense-grown cell monolayers, 'pan-cadherin' antibodies have reacted with a band at ~ 140 kDa, identified as N-cadherin by peptide fingerprinting of the immunoprecipitated protein, which for reasons not yet clear is modified or masked in immunolocalization experiments. The exact histological derivation of RMEC-1 cells is not known. However, the observations of several endothelial markers and the fact that all cells are rich in IFs containing vimentin and/or desmin, while only subpopulations also reveal IFs containing CKs 8 and 18, is suggestive of a mesenchymal, probably endothelial origin. We discuss the molecular relationship of this novel type of extended junction with other types of adhering junctions.

Original languageEnglish (US)
Pages (from-to)11-25
Number of pages15
JournalCell & Tissue Research
Volume294
Issue number1
DOIs
StatePublished - 1998

Fingerprint

Cell culture
Cadherins
Rats
Cell Culture Techniques
Cell Line
Desmosomes
Intermediate Filaments
Desmoplakins
gamma Catenin
Proteins
Vinculin
Catenins
Plakophilins
Desmocollins
Desmogleins
Zonula Occludens-1 Protein
Bearings (structural)
Actinin
Peptide Mapping
Desmin

Keywords

  • Adhering junctions
  • Cadherins
  • Desmoplakin
  • Desmosomes
  • Endothelial junctions
  • Plaque proteins
  • Protein ZO-1
  • Rat, cell culture

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

A novel type of adhering junction in an epithelioid tumorigenic rat cell culture line. / Schmelz, Monika; Way, Dennis L.; Borgs, Peter; Peitsch, Wiebke K.; Schmidt, Hannelore; Witte, Marlys H; Witte, Charles L.; Franke, Werner W.; Moll, Roland.

In: Cell & Tissue Research, Vol. 294, No. 1, 1998, p. 11-25.

Research output: Contribution to journalArticle

Schmelz, M, Way, DL, Borgs, P, Peitsch, WK, Schmidt, H, Witte, MH, Witte, CL, Franke, WW & Moll, R 1998, 'A novel type of adhering junction in an epithelioid tumorigenic rat cell culture line', Cell & Tissue Research, vol. 294, no. 1, pp. 11-25. https://doi.org/10.1007/s004410051152
Schmelz, Monika ; Way, Dennis L. ; Borgs, Peter ; Peitsch, Wiebke K. ; Schmidt, Hannelore ; Witte, Marlys H ; Witte, Charles L. ; Franke, Werner W. ; Moll, Roland. / A novel type of adhering junction in an epithelioid tumorigenic rat cell culture line. In: Cell & Tissue Research. 1998 ; Vol. 294, No. 1. pp. 11-25.
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