A PARACEST MRI contrast agent to detect enzyme activity

Byunghee Yoo, Marty D. Pagel

Research output: Contribution to journalArticle

150 Scopus citations

Abstract

A "smart" PARACEST MRI contrast agent was synthesized to detect caspase-3, which is an important biomarker in apoptosis. The well-known caspase-3 substrate, DEVD (Asp-Glu-Val-Asp), was elongated using the amino group on one sidearm of lanthanide ligand anchored on the polymer support. The amide of DEVD-(Tm-DOTA) showed a PARACEST effect with MR saturation at -51 ppm. DEVD-(Tm-DOTA) amide was successfully cleaved by caspase-3, exposing the free amine group, which showed PARACEST with saturation at +8 ppm. The enzymatic activity of caspase-3 can be detected by the change in PARACEST effect caused by this biotransformation.

Original languageEnglish (US)
Pages (from-to)14032-14033
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number43
DOIs
StatePublished - Nov 1 2006

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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