A plaque assay for the simian rotavirus SAII

E. M. Smith, M. Kolb Estes, D. Y. Graham, Charles P Gerba

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

Original languageEnglish (US)
Pages (from-to)513-519
Number of pages7
JournalJournal of General Virology
Volume43
Issue number3
StatePublished - 1979
Externally publishedYes

Fingerprint

Pancreatin
Rotavirus
Dextrans
Trypsin
Primates
Agar
Cell Line
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Smith, E. M., Kolb Estes, M., Graham, D. Y., & Gerba, C. P. (1979). A plaque assay for the simian rotavirus SAII. Journal of General Virology, 43(3), 513-519.

A plaque assay for the simian rotavirus SAII. / Smith, E. M.; Kolb Estes, M.; Graham, D. Y.; Gerba, Charles P.

In: Journal of General Virology, Vol. 43, No. 3, 1979, p. 513-519.

Research output: Contribution to journalArticle

Smith, EM, Kolb Estes, M, Graham, DY & Gerba, CP 1979, 'A plaque assay for the simian rotavirus SAII', Journal of General Virology, vol. 43, no. 3, pp. 513-519.
Smith EM, Kolb Estes M, Graham DY, Gerba CP. A plaque assay for the simian rotavirus SAII. Journal of General Virology. 1979;43(3):513-519.
Smith, E. M. ; Kolb Estes, M. ; Graham, D. Y. ; Gerba, Charles P. / A plaque assay for the simian rotavirus SAII. In: Journal of General Virology. 1979 ; Vol. 43, No. 3. pp. 513-519.
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