A plaque assay for the simian rotavirus SAII

E. M. Smith, M. Kolb Estes, D. Y. Graham, C. P. Gerba

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

Original languageEnglish (US)
Pages (from-to)513-519
Number of pages7
JournalJournal of General Virology
Volume43
Issue number3
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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