Abstract
A plasma lithography microengineered wound healing assay is developed to study architecture and injury dependences of endothelial cell migration. We study the migration of endothelial cell monolayers with dimensions from single cell width to millimeters mimicking various cardiovascular structures. Furthermore, we investigate the role of mechanical injury using wound healing assays that introduce different degrees of cell injury. Remarkably, the endothelial migration rate is robustly maintained in various circumstances: line patterns with different widths and different levels of mechanical injury. Our results also reveal that the number of leader cells, which guide the healing process, is proportional to the width of the structure suggesting a novel multicellular mechanism for regulating the endothelial wound healing process.
| Original language | English (US) |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 326-328 |
| Number of pages | 3 |
| Volume | 1 |
| State | Published - 2011 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: Oct 2 2011 → Oct 6 2011 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 10/2/11 → 10/6/11 |
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Keywords
- Architecture dependence
- Cell injury
- Endothelial cell
- Plasma lithography
- Wound healing
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
A plasma lithography microengineered assay for studying architecture dependent wound healing of endothelial cells. / Yang, Yongliang; Wong, Pak Kin.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. p. 326-328.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A plasma lithography microengineered assay for studying architecture dependent wound healing of endothelial cells
AU - Yang, Yongliang
AU - Wong, Pak Kin
PY - 2011
Y1 - 2011
N2 - A plasma lithography microengineered wound healing assay is developed to study architecture and injury dependences of endothelial cell migration. We study the migration of endothelial cell monolayers with dimensions from single cell width to millimeters mimicking various cardiovascular structures. Furthermore, we investigate the role of mechanical injury using wound healing assays that introduce different degrees of cell injury. Remarkably, the endothelial migration rate is robustly maintained in various circumstances: line patterns with different widths and different levels of mechanical injury. Our results also reveal that the number of leader cells, which guide the healing process, is proportional to the width of the structure suggesting a novel multicellular mechanism for regulating the endothelial wound healing process.
AB - A plasma lithography microengineered wound healing assay is developed to study architecture and injury dependences of endothelial cell migration. We study the migration of endothelial cell monolayers with dimensions from single cell width to millimeters mimicking various cardiovascular structures. Furthermore, we investigate the role of mechanical injury using wound healing assays that introduce different degrees of cell injury. Remarkably, the endothelial migration rate is robustly maintained in various circumstances: line patterns with different widths and different levels of mechanical injury. Our results also reveal that the number of leader cells, which guide the healing process, is proportional to the width of the structure suggesting a novel multicellular mechanism for regulating the endothelial wound healing process.
KW - Architecture dependence
KW - Cell injury
KW - Endothelial cell
KW - Plasma lithography
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=84883805629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883805629&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883805629
SN - 9781618395955
VL - 1
SP - 326
EP - 328
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ER -