A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups

Moon Kyoo Jang, D. Eric Anderson, Koenraad van Doorslaer, Alison A. Mcbride

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Papillomaviruses are a very successful group of viruses that replicate persistently in localized regions of the stratified epithelium of their specific host. Infection results in pathologies ranging from asymptomatic infection, benign warts, to malignant carcinomas. Despite this diversity, papillomavirus genomes are small (7-8 kbp) and contain at most eight genes. To sustain the complex papillomaviral life cycle, each viral protein has multiple functions and interacts with and manipulates a plethora of cellular proteins. In this study, we use tandem affinity purification and MS to identify host factors that interact with 11 different papillomavirus E2 proteins from diverse phylogenetic groups. The E2 proteins function in viral transcription and replication and correspondingly interact with host proteins involved in transcription, chromatin remodeling and modification, replication, and RNA processing.

Original languageEnglish (US)
Pages (from-to)2038-2050
Number of pages13
JournalProteomics
Volume15
Issue number12
DOIs
Publication statusPublished - Jun 1 2015
Externally publishedYes

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Keywords

  • BRD4
  • E2
  • HPV
  • Microbiology
  • Papillomavirus
  • Virus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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