A radical intermediate in bacillus subtilis quee during turnover with the substrate analogue 6-Carboxypterin

Jarett Wilcoxen, Nathan A. Bruender, Vahe Bandarian, R. David Britt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

7-Carboxy-7-deazaguanine (CDG) synthase (QueE), a member of the radical S-deoxyadenosyl-l-methionine (SAM) superfamily of enzymes, catalyzes a radical-mediated ring rearrangement required to convert 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) into CDG, forming the 7-dezapurine precursor to all pyrrolopyrimidine metabolites. Members of the radical SAM superfamily bind SAM to a [4Fe-4S] cluster, leveraging the reductive cleavage of SAM by the cluster to produce a highly reactive 5′-deoxyadenosyl radical which initiates chemistry by H atom abstraction from the substrate. QueE has recently been shown to use 6-carboxypterin (6-CP) as an alternative substrate, forming 6-deoxyadenosylpterin as the product. This reaction has been proposed to occur by radical addition between 5′-dAdo· and 6-CP, which upon oxidative decarboxylation yields the modified pterin. Here, we present spectroscopic evidence for a 6-CP-dAdo radical. The structure of this intermediate is determined by characterizing its electronic structure by continuous wave and pulse electron paramagnetic resonance spectroscopy.

Original languageEnglish (US)
Pages (from-to)1753-1759
Number of pages7
JournalJournal of the American Chemical Society
Volume140
Issue number5
DOIs
StatePublished - Feb 7 2018
Externally publishedYes

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Bacilli
Bacillus subtilis
Methionine
Substrates
Metabolites
Electronic structure
Paramagnetic resonance
Enzymes
Spectroscopy
Pterins
Atoms
Decarboxylation
Electron Spin Resonance Spectroscopy
Spectrum Analysis
6-carboxypterin
7-deazaguanine

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

A radical intermediate in bacillus subtilis quee during turnover with the substrate analogue 6-Carboxypterin. / Wilcoxen, Jarett; Bruender, Nathan A.; Bandarian, Vahe; Britt, R. David.

In: Journal of the American Chemical Society, Vol. 140, No. 5, 07.02.2018, p. 1753-1759.

Research output: Contribution to journalArticle

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