A rapid and specific high-performance liquid chromatographic assay for theophylline in biological fluids

J. Blanchard, S. Harvey, Wayne J Morgan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A rapid, specific high-performance liquid chromatographic analysis of theophylline in plasma, serum, and saliva is described. Proteins present in the biological samples are precipitated with 6% perchloric acid and the clear supernatant is chromatographed on a reversed-phase column. Only 100 μL of serum is required and concentrations as low as 0.07 μg/mL can be measured accurately. Other xanthines do not interfere in the assay. Within- and between-day variation is ≤ 2.2%. The method shows less bias and greater precision than the TDx (Abbott Diagnostics) procedure commonly used in clinical laboratories.

Original languageEnglish (US)
Pages (from-to)303-306
Number of pages4
JournalJournal of Chromatographic Science
Volume28
Issue number6
StatePublished - 1990

Fingerprint

Xanthines
Clinical laboratories
High performance liquid chromatography
Theophylline
Assays
Plasmas
Fluids
Liquids
Serum
Saliva
Proteins
High Pressure Liquid Chromatography
Perchloric Acid

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

A rapid and specific high-performance liquid chromatographic assay for theophylline in biological fluids. / Blanchard, J.; Harvey, S.; Morgan, Wayne J.

In: Journal of Chromatographic Science, Vol. 28, No. 6, 1990, p. 303-306.

Research output: Contribution to journalArticle

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