A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution

Francisco G. Nobrega, Carol L Dieckmann, Alexander Tzagoloff

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

Original languageEnglish (US)
Pages (from-to)141-145
Number of pages5
JournalAnalytical Biochemistry
Volume131
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

DNA Probes
Sepharose
Visualization
RNA
Autoradiography
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution. / Nobrega, Francisco G.; Dieckmann, Carol L; Tzagoloff, Alexander.

In: Analytical Biochemistry, Vol. 131, No. 1, 1983, p. 141-145.

Research output: Contribution to journalArticle

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