A rapid procedure for the isolation of C0t-1 DNA from plants

Michael S. Zwick, Robert E. Hanson, Thomas D. McKnight, M. Nurul Islam-Faridi, David M. Stelly, Rod A. Wing, H. James Price

Research output: Contribution to journalArticlepeer-review

171 Scopus citations

Abstract

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or 'blocking' of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.

Original languageEnglish (US)
Pages (from-to)138-142
Number of pages5
JournalGenome
Volume40
Issue number1
DOIs
StatePublished - 1997

Keywords

  • BACs
  • Ct-1 DNA
  • In situ hybridization
  • Plants

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'A rapid procedure for the isolation of C<sub>0</sub>t-1 DNA from plants'. Together they form a unique fingerprint.

Cite this