A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus

L. C. Papayiannis, J. K. Brown, N. A. Seraphides, M. Hadjistylli, N. Ioannou, N. I. Katis

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A real-time PCR assay based on TaqMan® technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005-2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan® PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene. Two biotypes, B and Q, were identified in the collected plant species on the island. The real-time PCR and RFLP assay consistently yielded the same results, although the real-time assay was more sensitive and less time consuming. Phylogenetic analysis of the mtCOI DNA sequences corroborated the identity of the B and Q biotypes 100% of the time and by phylogenetic analysis the haplotypes grouped, as expected, in the major North African-Mediterranean-Middle Eastern clade of the B. tabaci complex.

Original languageEnglish (US)
Pages (from-to)573-582
Number of pages10
JournalBulletin of Entomological Research
Volume99
Issue number6
DOIs
StatePublished - Dec 1 2009

Keywords

  • Bemisia tabaci complex
  • Haplotype
  • Mitochondrial COI gene
  • RFLP analysis
  • Real-time PCR

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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