A reassessment of structure-function relationships in glucagon. Glucagon1-21 is a full agonist

D. E. Wright, Victor J Hruby, M. Rodbell

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Glucagon1-21 has been prepared by treating native glucagon with carboxypeptidase A. Purified glucagon1-21 did not contain detectable methionine (<0.001 residue/mol) and the activity of the compound did not change after treatment with cyanogen bromide as has been shown with native glucagon. Glucagon1-21 stimulates hepatic adenylate cyclase activity to the same extent as native glucagon but with 0.1% the potency. Glucagon1-21 also displayed 0.1% the binding affinity of native glucagon to the glucagon receptor in hepatic membranes. Glucagon22-29 alone or in combination with glucagon1-21 did not activate adenylate cyclase or displace 125I-glucagon from its receptor. The finding that glucagon1-21 is a full agonist on adenylate cyclase is discussed in relation to the structure-function relationships required for the biological action of glucagon.

Original languageEnglish (US)
Pages (from-to)6338-6340
Number of pages3
JournalJournal of Biological Chemistry
Volume253
Issue number18
StatePublished - 1978
Externally publishedYes

Fingerprint

Glucagon
Glucagon Receptors
Adenylyl Cyclases
Carboxypeptidases A
Cyanogen Bromide
Liver
Methionine
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

A reassessment of structure-function relationships in glucagon. Glucagon1-21 is a full agonist. / Wright, D. E.; Hruby, Victor J; Rodbell, M.

In: Journal of Biological Chemistry, Vol. 253, No. 18, 1978, p. 6338-6340.

Research output: Contribution to journalArticle

@article{04d33529fd1446598f356ad90e884157,
title = "A reassessment of structure-function relationships in glucagon. Glucagon1-21 is a full agonist",
abstract = "Glucagon1-21 has been prepared by treating native glucagon with carboxypeptidase A. Purified glucagon1-21 did not contain detectable methionine (<0.001 residue/mol) and the activity of the compound did not change after treatment with cyanogen bromide as has been shown with native glucagon. Glucagon1-21 stimulates hepatic adenylate cyclase activity to the same extent as native glucagon but with 0.1{\%} the potency. Glucagon1-21 also displayed 0.1{\%} the binding affinity of native glucagon to the glucagon receptor in hepatic membranes. Glucagon22-29 alone or in combination with glucagon1-21 did not activate adenylate cyclase or displace 125I-glucagon from its receptor. The finding that glucagon1-21 is a full agonist on adenylate cyclase is discussed in relation to the structure-function relationships required for the biological action of glucagon.",
author = "Wright, {D. E.} and Hruby, {Victor J} and M. Rodbell",
year = "1978",
language = "English (US)",
volume = "253",
pages = "6338--6340",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "18",

}

TY - JOUR

T1 - A reassessment of structure-function relationships in glucagon. Glucagon1-21 is a full agonist

AU - Wright, D. E.

AU - Hruby, Victor J

AU - Rodbell, M.

PY - 1978

Y1 - 1978

N2 - Glucagon1-21 has been prepared by treating native glucagon with carboxypeptidase A. Purified glucagon1-21 did not contain detectable methionine (<0.001 residue/mol) and the activity of the compound did not change after treatment with cyanogen bromide as has been shown with native glucagon. Glucagon1-21 stimulates hepatic adenylate cyclase activity to the same extent as native glucagon but with 0.1% the potency. Glucagon1-21 also displayed 0.1% the binding affinity of native glucagon to the glucagon receptor in hepatic membranes. Glucagon22-29 alone or in combination with glucagon1-21 did not activate adenylate cyclase or displace 125I-glucagon from its receptor. The finding that glucagon1-21 is a full agonist on adenylate cyclase is discussed in relation to the structure-function relationships required for the biological action of glucagon.

AB - Glucagon1-21 has been prepared by treating native glucagon with carboxypeptidase A. Purified glucagon1-21 did not contain detectable methionine (<0.001 residue/mol) and the activity of the compound did not change after treatment with cyanogen bromide as has been shown with native glucagon. Glucagon1-21 stimulates hepatic adenylate cyclase activity to the same extent as native glucagon but with 0.1% the potency. Glucagon1-21 also displayed 0.1% the binding affinity of native glucagon to the glucagon receptor in hepatic membranes. Glucagon22-29 alone or in combination with glucagon1-21 did not activate adenylate cyclase or displace 125I-glucagon from its receptor. The finding that glucagon1-21 is a full agonist on adenylate cyclase is discussed in relation to the structure-function relationships required for the biological action of glucagon.

UR - http://www.scopus.com/inward/record.url?scp=0018123477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018123477&partnerID=8YFLogxK

M3 - Article

VL - 253

SP - 6338

EP - 6340

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 18

ER -