A redox equilibrator for the preparation of cytochrome oxidase of mixed valence states and intermediate compounds for X-ray synchrotron studies

Britton Chance; Jonathan Moore; Linda Powers; Y. Ching

doi:10.1016/0003-2697(82)90034-3

A redox equilibrator for the preparation of cytochrome oxidase of mixed valence states and intermediate compounds for X-ray synchrotron studies

Britton Chance, Jonathan Moore, Linda Powers, Y. Ching

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A redox titrator for the preparation of small volumes of highly concentrated biological samples for X-ray synchrotron studies provides for the redox equilibration at temperatures of 40 to -30°C. Anaerobic transfer directly to the X-ray sample chamber and mixing of an additional reagent just prior to freeze-trapping of the redox-equilibrated sample are provided.

Original language	English (US)
Pages (from-to)	239-247
Number of pages	9
Journal	Analytical Biochemistry
Volume	124
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(82)90034-3
State	Published - Aug 1982
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{8620405666e54daa849bb7e21a96c0a4,

title = "A redox equilibrator for the preparation of cytochrome oxidase of mixed valence states and intermediate compounds for X-ray synchrotron studies",

abstract = "A redox titrator for the preparation of small volumes of highly concentrated biological samples for X-ray synchrotron studies provides for the redox equilibration at temperatures of 40 to -30°C. Anaerobic transfer directly to the X-ray sample chamber and mixing of an additional reagent just prior to freeze-trapping of the redox-equilibrated sample are provided.",

author = "Britton Chance and Jonathan Moore and Linda Powers and Y. Ching",

note = "Funding Information: This research was supported in part by NIH Grants GM 27476, GM 27308, HL 18708, and HL 28385, NSF Grant PM-80-26684, and SSRL Project 423B, through the Division of Materials Research of the NSF and the Biotechnology Resource Program, Division of Research Resources, of the NIH in cooperation with the Department of Energy.",

year = "1982",

month = aug,

doi = "10.1016/0003-2697(82)90034-3",

language = "English (US)",

volume = "124",

pages = "239--247",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - A redox equilibrator for the preparation of cytochrome oxidase of mixed valence states and intermediate compounds for X-ray synchrotron studies

AU - Chance, Britton

AU - Moore, Jonathan

AU - Powers, Linda

AU - Ching, Y.

N1 - Funding Information: This research was supported in part by NIH Grants GM 27476, GM 27308, HL 18708, and HL 28385, NSF Grant PM-80-26684, and SSRL Project 423B, through the Division of Materials Research of the NSF and the Biotechnology Resource Program, Division of Research Resources, of the NIH in cooperation with the Department of Energy.

PY - 1982/8

Y1 - 1982/8

N2 - A redox titrator for the preparation of small volumes of highly concentrated biological samples for X-ray synchrotron studies provides for the redox equilibration at temperatures of 40 to -30°C. Anaerobic transfer directly to the X-ray sample chamber and mixing of an additional reagent just prior to freeze-trapping of the redox-equilibrated sample are provided.

AB - A redox titrator for the preparation of small volumes of highly concentrated biological samples for X-ray synchrotron studies provides for the redox equilibration at temperatures of 40 to -30°C. Anaerobic transfer directly to the X-ray sample chamber and mixing of an additional reagent just prior to freeze-trapping of the redox-equilibrated sample are provided.

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U2 - 10.1016/0003-2697(82)90034-3

DO - 10.1016/0003-2697(82)90034-3

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VL - 124

SP - 239

EP - 247

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -