A redox equilibrator for the preparation of cytochrome oxidase of mixed valence states and intermediate compounds for X-ray synchrotron studies

Britton Chance, Jonathan Moore, Linda Powers, Y. Ching

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A redox titrator for the preparation of small volumes of highly concentrated biological samples for X-ray synchrotron studies provides for the redox equilibration at temperatures of 40 to -30°C. Anaerobic transfer directly to the X-ray sample chamber and mixing of an additional reagent just prior to freeze-trapping of the redox-equilibrated sample are provided.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalAnalytical Biochemistry
Volume124
Issue number2
DOIs
StatePublished - Aug 1982

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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