A renal-like organic anion transport system in the ciliary epithelium of the bovine and human eye

Jonghwa Lee, Mohammad - Shahidullah, Adam Hotchkiss, Miguel Coca-Prados, Nicholas A Delamere, Ryan M. Pelis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The purpose of this study was to determine the direction of organic anion (OA) transport across the ciliary body and the transport proteins that may contribute. Transport of several OAs across the bovine ciliary body was examined using ciliary body sections mounted in Ussing chambers and a perfused eye preparation. Microarray, reverse-transcription polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry were used to examine OA transporter expression in human ocular tissues. Microarray analysis showed that many OA transporters common to other barrier epithelia are expressed in ocular tissues. mRNA (RT-PCR) and protein (immunoblotting) for OAT1, OAT3, NaDC3, and MRP4 were detected in extracts of the human ciliary body from several donors. OAT1 and OAT3 localized to basolateral membranes of nonpigmented epithelial cells and MRP4 to basolateral membranes of pigmented cells in the human eye. Para-aminohippurate (PAH) and estrone-3-sulfate transport across the bovine ciliary body in the Ussing chambers was greater in the aqueous humor-to-blood direction than in the blood-to-aqueous humor direction, and active. There was little net directional movement of cidofovir. Probenecid (0.1 mM) or novobiocin (0.1 mM) added to the aqueous humor side of the tissue, or MK571 (5-(3-(2-(7-chloroquinolin-2-yl)ethenyl)phenyl)-8-dimethylcarbamyl-4,6-dithiaoctanoic acid; 0.1 mM) added to the blood side significantly reduced net active PAH transport. The rate of 6-carboxyfluorescein elimination from the aqueous humor of the perfused eye was reduced 80% when novobiocin (0.1 mM) was present in the aqueous humor. These data indicate that the ciliary body expresses a variety of OA transporters, including those common to the kidney. They are likely involved in clearing potentially harmful endobiotic and xenobiotic OAs from the eye.

Original languageEnglish (US)
Pages (from-to)697-705
Number of pages9
JournalMolecular Pharmacology
Volume87
Issue number4
DOIs
StatePublished - Apr 1 2015

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Ciliary Body
Aqueous Humor
Anions
Epithelium
Organic Anion Transporters
Kidney
Novobiocin
Immunoblotting
Organic Anion Transport Protein 1
Reverse Transcription
Probenecid
Polymerase Chain Reaction
Xenobiotics
Microarray Analysis
Human Body
Carrier Proteins
Epithelial Cells
Immunohistochemistry
Cell Membrane
Messenger RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A renal-like organic anion transport system in the ciliary epithelium of the bovine and human eye. / Lee, Jonghwa; Shahidullah, Mohammad -; Hotchkiss, Adam; Coca-Prados, Miguel; Delamere, Nicholas A; Pelis, Ryan M.

In: Molecular Pharmacology, Vol. 87, No. 4, 01.04.2015, p. 697-705.

Research output: Contribution to journalArticle

Lee, Jonghwa ; Shahidullah, Mohammad - ; Hotchkiss, Adam ; Coca-Prados, Miguel ; Delamere, Nicholas A ; Pelis, Ryan M. / A renal-like organic anion transport system in the ciliary epithelium of the bovine and human eye. In: Molecular Pharmacology. 2015 ; Vol. 87, No. 4. pp. 697-705.
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