A repressor region in the human β-myosin heavy chain gene that has a partial position dependency

J. G. Edwards; J. J. Bahl; I. Flink; J. Milavetz; Steven Goldman; E. Morkin

doi:10.1016/0006-291X(92)91586-F

A repressor region in the human β-myosin heavy chain gene that has a partial position dependency

J. G. Edwards, J. J. Bahl, I. Flink, J. Milavetz, Steven Goldman, E. Morkin

Medicine

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22 Scopus citations

Abstract

Expression of the human β-myosin heavy chain (βMHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5′-TTGGTGGTCGTGGTCAGT-3′) of the human βMHC gene that is conserved among the rat, rabbit, and human βMHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5′ but not 3′ to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.

Original language	English (US)
Pages (from-to)	504-510
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	189
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(92)91586-F
Publication status	Published - Nov 30 1992

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ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Edwards, J. G., Bahl, J. J., Flink, I., Milavetz, J., Goldman, S., & Morkin, E. (1992). A repressor region in the human β-myosin heavy chain gene that has a partial position dependency. Biochemical and Biophysical Research Communications, 189(1), 504-510. https://doi.org/10.1016/0006-291X(92)91586-F

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10.1016/0006-291X(92)91586-F