A repressor region in the human β-myosin heavy chain gene that has a partial position dependency

J. G. Edwards, J. J. Bahl, I. Flink, J. Milavetz, S. Goldman, E. Morkin

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Expression of the human β-myosin heavy chain (βMHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5′-TTGGTGGTCGTGGTCAGT-3′) of the human βMHC gene that is conserved among the rat, rabbit, and human βMHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5′ but not 3′ to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.

Original languageEnglish (US)
Pages (from-to)504-510
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume189
Issue number1
DOIs
StatePublished - Nov 30 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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