Abstract
Expression of the human β-myosin heavy chain (βMHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5′-TTGGTGGTCGTGGTCAGT-3′) of the human βMHC gene that is conserved among the rat, rabbit, and human βMHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5′ but not 3′ to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 504-510 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 189 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 30 1992 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A repressor region in the human β-myosin heavy chain gene that has a partial position dependency. / Edwards, J. G.; Bahl, J. J.; Flink, I.; Milavetz, J.; Goldman, Steven; Morkin, E.
In: Biochemical and Biophysical Research Communications, Vol. 189, No. 1, 30.11.1992, p. 504-510.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A repressor region in the human β-myosin heavy chain gene that has a partial position dependency
AU - Edwards, J. G.
AU - Bahl, J. J.
AU - Flink, I.
AU - Milavetz, J.
AU - Goldman, Steven
AU - Morkin, E.
PY - 1992/11/30
Y1 - 1992/11/30
N2 - Expression of the human β-myosin heavy chain (βMHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5′-TTGGTGGTCGTGGTCAGT-3′) of the human βMHC gene that is conserved among the rat, rabbit, and human βMHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5′ but not 3′ to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.
AB - Expression of the human β-myosin heavy chain (βMHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5′-TTGGTGGTCGTGGTCAGT-3′) of the human βMHC gene that is conserved among the rat, rabbit, and human βMHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5′ but not 3′ to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.
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U2 - 10.1016/0006-291X(92)91586-F
DO - 10.1016/0006-291X(92)91586-F
M3 - Article
VL - 189
SP - 504
EP - 510
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -