A repressor region in the human β-myosin heavy chain gene that has a partial position dependency

J. G. Edwards; J. J. Bahl; I. Flink; J. Milavetz; S. Goldman; E. Morkin

doi:10.1016/0006-291X(92)91586-F

A repressor region in the human β-myosin heavy chain gene that has a partial position dependency

J. G. Edwards, J. J. Bahl, I. Flink, J. Milavetz, S. Goldman, E. Morkin

Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Expression of the human β-myosin heavy chain (βMHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5′-TTGGTGGTCGTGGTCAGT-3′) of the human βMHC gene that is conserved among the rat, rabbit, and human βMHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5′ but not 3′ to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.

Original language	English (US)
Pages (from-to)	504-510
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	189
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(92)91586-F
State	Published - Nov 30 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AU - Goldman, S.

AU - Morkin, E.

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