A review of genetic engineering biotechnologies for enhanced chronic wound healing

John W. Sessions, David G. Armstrong, Sandra Hope, Brian D. Jensen

Research output: Research - peer-reviewReview article

  • 3 Citations

Abstract

Traditional methods for addressing chronic wounds focus on correcting dysfunction by controlling extracellular elements. This review highlights technologies that take a different approach – enhancing chronic wound healing by genetic modification to wound beds. Featured cutaneous transduction/transfection methods include viral modalities (ie adenoviruses, adeno-associated viruses, retroviruses and lentiviruses) and conventional non-viral modalities (ie naked DNA injections, microseeding, liposomal reagents, particle bombardment and electroporation). Also explored are emerging technologies, focusing on the exciting capabilities of wound diagnostics such as pyrosequencing as well as site-specific nuclease editing tools such as CRISPR-Cas9 used to both transiently and permanently genetically modify resident wound bed cells. Additionally, new non-viral transfection methods (ie conjugated nanoparticles, multi-electrode arrays, and microfabricated needles and nanowires) are discussed that can potentially facilitate more efficient and safe transgene delivery to skin but also represent significant advances broadly to tissue regeneration research.

LanguageEnglish (US)
Pages179-185
Number of pages7
JournalExperimental Dermatology
Volume26
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Genetic Engineering
Biotechnology
Wound Healing
Wounds and Injuries
Genetic engineering
Transfection
Technology
Skin
Clustered Regularly Interspaced Short Palindromic Repeats
Tissue regeneration
Viruses
Needles
Nanowires
Nanoparticles
Electrodes
DNA
Dependovirus
Lentivirus
Electroporation
Retroviridae

Keywords

  • chronic wound healing
  • CRISPR-Cas9
  • pyrosequencing
  • transfection nanotechnology
  • viral transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

A review of genetic engineering biotechnologies for enhanced chronic wound healing. / Sessions, John W.; Armstrong, David G.; Hope, Sandra; Jensen, Brian D.

In: Experimental Dermatology, Vol. 26, No. 2, 01.02.2017, p. 179-185.

Research output: Research - peer-reviewReview article

Sessions, John W. ; Armstrong, David G. ; Hope, Sandra ; Jensen, Brian D./ A review of genetic engineering biotechnologies for enhanced chronic wound healing. In: Experimental Dermatology. 2017 ; Vol. 26, No. 2. pp. 179-185
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