A role for polypyrimidine tract binding protein in the establishment of focal adhesions

Ivan Babic, Shalini Sharma, Douglas L. Black

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Polypyrimidine tract binding protein (PTB) is a widely expressed RNA binding protein. In the nucleus PTB regulates the splicing of alternative exons, while in the cytoplasm it can affect mRNA stability, translation, and localization. Here we demonstrate that PTB transiently localizes to the cytoplasm and to protrusions in the cellular edge of mouse embryo fibroblasts during adhesion to fibronectin and the early stages of cell spreading. This cytoplasmic PTB is associated with transcripts encoding the focal adhesion scaffolding proteins vinculin and alpha-actinin 4. We demonstrate that vinculin mRNA colocalizes with PTB to cytoplasmic protrusions and that PTB depletion reduces vinculin mRNA at the cellular edge and limits the size of focal adhesions. The loss of PTB also alters cell morphology and limits the ability of cells to spread after adhesion. These data indicate that during the initial stages of cell adhesion, PTB shuttles from the nucleus to the cytoplasm and influences focal adhesion formation through coordinated control of scaffolding protein mRNAs.

Original languageEnglish (US)
Pages (from-to)5564-5577
Number of pages14
JournalMolecular and Cellular Biology
Volume29
Issue number20
DOIs
StatePublished - Oct 2009
Externally publishedYes

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Polypyrimidine Tract-Binding Protein
Focal Adhesions
Vinculin
Cytoplasm
Messenger RNA
Cell Surface Extensions
Protein Splicing
Actinin
RNA-Binding Proteins
RNA Stability
Protein Biosynthesis
Fibronectins
Cell Adhesion
Exons
Proteins
Embryonic Structures
Fibroblasts

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A role for polypyrimidine tract binding protein in the establishment of focal adhesions. / Babic, Ivan; Sharma, Shalini; Black, Douglas L.

In: Molecular and Cellular Biology, Vol. 29, No. 20, 10.2009, p. 5564-5577.

Research output: Contribution to journalArticle

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