A route to de novo domestication of wild allotetraploid rice

Hong Yu, Tao Lin, Xiangbing Meng, Huilong Du, Jingkun Zhang, Guifu Liu, Mingjiang Chen, Yanhui Jing, Liquan Kou, Xiuxiu Li, Qiang Gao, Yan Liang, Xiangdong Liu, Zhilan Fan, Yuntao Liang, Zhukuan Cheng, Mingsheng Chen, Zhixi Tian, Yonghong Wang, Chengcai ChuJianru Zuo, Jianmin Wan, Qian Qian, Bin Han, Andrea Zuccolo, Rod A. Wing, Caixia Gao, Chengzhi Liang, Jiayang Li

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired because of its advantages in genome buffering, vigorousness, and environmental robustness. However, a workable route remains elusive. Here, we describe a practical strategy, namely de novo domestication of wild allotetraploid rice. By screening allotetraploid wild rice inventory, we identified one genotype of Oryza alta (CCDD), polyploid rice 1 (PPR1), and established two important resources for its de novo domestication: (1) an efficient tissue culture, transformation, and genome editing system and (2) a high-quality genome assembly discriminated into two subgenomes of 12 chromosomes apiece. With these resources, we show that six agronomically important traits could be rapidly improved by editing O. alta homologs of the genes controlling these traits in diploid rice. Our results demonstrate the possibility that de novo domesticated allotetraploid rice can be developed into a new staple cereal to strengthen world food security. Li and colleagues developed a breeding route to de novo domestication of wild allotetraploid rice that provides a rational strategy for creating novel crops and generated a series of allotetraploid rice lines edited in domestication-related and agronomically important genes.

Original languageEnglish (US)
Pages (from-to)1156-1170.e14
JournalCell
Volume184
Issue number5
DOIs
StatePublished - Mar 4 2021

Keywords

  • Oryza alta
  • comparative genomics
  • de novo domestication
  • genetic transformation
  • genome
  • genome editing
  • genome evolution
  • polyploid
  • structural variation
  • tissue culture

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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