A sensitive microassay for the murine alternative compliment pathway

Keith A. Joiner, Robert Shahon, Jeffrey A. Gelfand

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses 51Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg2+ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8-10-fold, enabling the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains when alternative complement pathway activity of serum from various inbred murine lines was measured.

Original languageEnglish (US)
Pages (from-to)283-290
Number of pages8
JournalJournal of Immunological Methods
Volume31
Issue number3-4
DOIs
StatePublished - Dec 27 1979
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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