A serum-free medium that supports the growth of cultured skeletal muscle satellite cells

Ronald E. Allen, Michael V. Dodson, Lynda S. Luiten, Linda K. Boxhorn

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.

Original languageEnglish (US)
Pages (from-to)636-640
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume21
Issue number11
DOIs
StatePublished - Nov 1985

Keywords

  • fetuin
  • fibroblast growth factor
  • insulin
  • myogenesis
  • satellite cell
  • serum-free

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Fingerprint Dive into the research topics of 'A serum-free medium that supports the growth of cultured skeletal muscle satellite cells'. Together they form a unique fingerprint.

  • Cite this