A simple and rapid radio-receptor assay for the estimation of acetylcholine

Frederick J. Ehlert, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The high potency with which acetylcholine (ACh) inhibits the binding of the specific muscarinic agonist, [3H]cis methyldioxolane ([3H]CD), has provided the basis for the development of a radio-receptor assay for estimation of ACh. A synaptosomal preparation of the rat cerebral cortex was used as a source of muscarinic receptors. When binding assays were run at 0°C, the IC50 value of ACh was approximately 5 × 10-9 M, which corresponds to 2.5 - 10 pmoles of ACh, depending upon the assay volume. The ACh content of the rat cerebral cortex and corpus striatum was measured following fast microwave irradiation. By measuring the displacement of [3H]CD binding caused by aliquots of the supernatant from tissue homogenates and comparing the displacement values with an ACh standard curve, the ACh content of the cerebral cortex and corpus striatum was calculated to be 19 and 55 nmoles/g wet tissue weight, respectively.

Original languageEnglish (US)
Pages (from-to)347-354
Number of pages8
JournalLife Sciences
Volume31
Issue number4
DOIs
StatePublished - Jul 26 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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