A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S. R. Farrah, S. M. Goyal, C. P. Gerba, R. H. Conklin, C. Wallis, J. L. Melnick, H. L. DuPont

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

Original languageEnglish (US)
Pages (from-to)56-59
Number of pages4
JournalUnknown Journal
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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