A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S. R. Farrah, S. M. Goyal, Charles P Gerba, R. H. Conklin, C. Wallis, J. L. Melnick, H. L. DuPont

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

Original languageEnglish (US)
Pages (from-to)56-59
Number of pages4
JournalIntervirology
Volume9
Issue number1
StatePublished - 1978
Externally publishedYes

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Enterovirus
Rotavirus
Cell Culture Techniques
Adsorption
Membranes
Viruses
Fluorocarbons
Viral Structures
Buffers

ASJC Scopus subject areas

  • Virology

Cite this

Farrah, S. R., Goyal, S. M., Gerba, C. P., Conklin, R. H., Wallis, C., Melnick, J. L., & DuPont, H. L. (1978). A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters. Intervirology, 9(1), 56-59.

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters. / Farrah, S. R.; Goyal, S. M.; Gerba, Charles P; Conklin, R. H.; Wallis, C.; Melnick, J. L.; DuPont, H. L.

In: Intervirology, Vol. 9, No. 1, 1978, p. 56-59.

Research output: Contribution to journalArticle

Farrah, SR, Goyal, SM, Gerba, CP, Conklin, RH, Wallis, C, Melnick, JL & DuPont, HL 1978, 'A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters', Intervirology, vol. 9, no. 1, pp. 56-59.
Farrah, S. R. ; Goyal, S. M. ; Gerba, Charles P ; Conklin, R. H. ; Wallis, C. ; Melnick, J. L. ; DuPont, H. L. / A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters. In: Intervirology. 1978 ; Vol. 9, No. 1. pp. 56-59.
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