A simple method for isolation of megabase DNA from cotton

Xinping Zhao, Hong Bin Zhang, Rod A. Wing, Andrew H. Paterson

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalPlant Molecular Biology Reporter
Volume12
Issue number2
DOIs
StatePublished - Jun 1 1994
Externally publishedYes

Keywords

  • Gossypium
  • genome analysis
  • high molecular weight DNA
  • physical mapping
  • pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Fingerprint Dive into the research topics of 'A simple method for isolation of megabase DNA from cotton'. Together they form a unique fingerprint.

  • Cite this