A simple method for isolation of megabase DNA from cotton

Xinping Zhao, Hong Bin Zhang, Rod A Wing, Andrew H. Paterson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalPlant Molecular Biology Reporter
Volume12
Issue number2
DOIs
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

isolation techniques
Cotton
cotton
physical chromosome mapping
Cloning
DNA
Sepharose
agarose
molecular cloning
Molecular weight
molecular weight
Microspheres
Organism Cloning
Molecular Weight
methodology

Keywords

  • genome analysis
  • Gossypium
  • high molecular weight DNA
  • physical mapping
  • pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

A simple method for isolation of megabase DNA from cotton. / Zhao, Xinping; Zhang, Hong Bin; Wing, Rod A; Paterson, Andrew H.

In: Plant Molecular Biology Reporter, Vol. 12, No. 2, 06.1994, p. 110-115.

Research output: Contribution to journalArticle

Zhao, Xinping ; Zhang, Hong Bin ; Wing, Rod A ; Paterson, Andrew H. / A simple method for isolation of megabase DNA from cotton. In: Plant Molecular Biology Reporter. 1994 ; Vol. 12, No. 2. pp. 110-115.
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