A simple photo-affinity labeling protocol

Hong Yu Li, Ying Liu, Kan Fang, Koji Nakanishi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

After photo-crosslinking and proteolysis of a photoaffinity labeled ligand to its receptor, the ester group incorporated in the ligand is cleaved by an amine with specific functionality, which displaces the bulk of the ligand from the peptide fragments, and facilitates subsequent tandem mass spectrometric sequencing.

Original languageEnglish (US)
Pages (from-to)365-366
Number of pages2
JournalChemical Communications
Issue number4
StatePublished - Feb 21 1999
Externally publishedYes

Fingerprint

Labeling
Ligands
Proteolysis
Peptide Fragments
Crosslinking
Peptides
Amines
Esters

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Li, H. Y., Liu, Y., Fang, K., & Nakanishi, K. (1999). A simple photo-affinity labeling protocol. Chemical Communications, (4), 365-366.

A simple photo-affinity labeling protocol. / Li, Hong Yu; Liu, Ying; Fang, Kan; Nakanishi, Koji.

In: Chemical Communications, No. 4, 21.02.1999, p. 365-366.

Research output: Contribution to journalArticle

Li, HY, Liu, Y, Fang, K & Nakanishi, K 1999, 'A simple photo-affinity labeling protocol', Chemical Communications, no. 4, pp. 365-366.
Li HY, Liu Y, Fang K, Nakanishi K. A simple photo-affinity labeling protocol. Chemical Communications. 1999 Feb 21;(4):365-366.
Li, Hong Yu ; Liu, Ying ; Fang, Kan ; Nakanishi, Koji. / A simple photo-affinity labeling protocol. In: Chemical Communications. 1999 ; No. 4. pp. 365-366.
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