A simple photo-affinity labeling protocol

Hong Yu Li, Ying Liu, Kan Fang, Koji Nakanishi

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

After photo-crosslinking and proteolysis of a photoaffinity labeled ligand to its receptor, the ester group incorporated in the ligand is cleaved by an amine with specific functionality, which displaces the bulk of the ligand from the peptide fragments, and facilitates subsequent tandem mass spectrometric sequencing.

Original languageEnglish (US)
Pages (from-to)365-366
Number of pages2
JournalChemical Communications
Issue number4
Publication statusPublished - Feb 21 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Li, H. Y., Liu, Y., Fang, K., & Nakanishi, K. (1999). A simple photo-affinity labeling protocol. Chemical Communications, (4), 365-366.