A simplified gas chromatographic method for quantifying the sevoflurane metabolite hexafluoroisopropanol

S. E. Morgan, E. J. Frink, A Jay Gandolfi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: The results of sevoflurane biotransformation (fluoromethyl- 1,1,1,3,3,3,-hexafluoro-2-propyl ether) to inorganic fluoride have been examined. However, these investigations have lacked a simplified assay for determining the primary organic metabolite, hexafluoroisopropanol. Previous attempts have involved extensive extraction steps, complicated derivatization techniques, or sophisticated detectors. Methods: After enzymatic hydrolysis of conjugates, hexafluoroisopropanol is detected readily using a head space gas chromatographic analysis with a flame ionization detector. Results: The gas chromatographic technique was linear from 10 to 800 μM with a correlation coefficient of 0.999. The detection limit was 10 μM in urine and 25 μM in blood. Conclusions: This simplified approach does not require the extraction, derivatization, or mass spectrometric detectors of previous methods. As sevoflurane utilization and research increases, this assay should allow for a variety of laboratory and clinical disposition studies to be performed.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalAnesthesiology
Volume80
Issue number1
StatePublished - 1994

Fingerprint

Gases
Flame Ionization
Biotransformation
Fluorides
Gas Chromatography
Limit of Detection
Hydrolysis
Head
Urine
Research
sevoflurane
hexafluoroisopropanol
propyl ether
Clinical Studies

Keywords

  • Anesthetic gases: sevoflurane
  • Biotransformation: metabolite
  • Hexafluoroisopropanol
  • Instrumentation: gas chromatography

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

A simplified gas chromatographic method for quantifying the sevoflurane metabolite hexafluoroisopropanol. / Morgan, S. E.; Frink, E. J.; Gandolfi, A Jay.

In: Anesthesiology, Vol. 80, No. 1, 1994, p. 201-205.

Research output: Contribution to journalArticle

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N2 - Background: The results of sevoflurane biotransformation (fluoromethyl- 1,1,1,3,3,3,-hexafluoro-2-propyl ether) to inorganic fluoride have been examined. However, these investigations have lacked a simplified assay for determining the primary organic metabolite, hexafluoroisopropanol. Previous attempts have involved extensive extraction steps, complicated derivatization techniques, or sophisticated detectors. Methods: After enzymatic hydrolysis of conjugates, hexafluoroisopropanol is detected readily using a head space gas chromatographic analysis with a flame ionization detector. Results: The gas chromatographic technique was linear from 10 to 800 μM with a correlation coefficient of 0.999. The detection limit was 10 μM in urine and 25 μM in blood. Conclusions: This simplified approach does not require the extraction, derivatization, or mass spectrometric detectors of previous methods. As sevoflurane utilization and research increases, this assay should allow for a variety of laboratory and clinical disposition studies to be performed.

AB - Background: The results of sevoflurane biotransformation (fluoromethyl- 1,1,1,3,3,3,-hexafluoro-2-propyl ether) to inorganic fluoride have been examined. However, these investigations have lacked a simplified assay for determining the primary organic metabolite, hexafluoroisopropanol. Previous attempts have involved extensive extraction steps, complicated derivatization techniques, or sophisticated detectors. Methods: After enzymatic hydrolysis of conjugates, hexafluoroisopropanol is detected readily using a head space gas chromatographic analysis with a flame ionization detector. Results: The gas chromatographic technique was linear from 10 to 800 μM with a correlation coefficient of 0.999. The detection limit was 10 μM in urine and 25 μM in blood. Conclusions: This simplified approach does not require the extraction, derivatization, or mass spectrometric detectors of previous methods. As sevoflurane utilization and research increases, this assay should allow for a variety of laboratory and clinical disposition studies to be performed.

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