A single molecule scaffold for the maize genome

Shiguo Zhou, Fusheng Wei, John Nguyen, Mike Bechner, Konstantinos Potamousis, Steve Goldstein, Louise Pape, Michael R. Mehan, Chris Churas, Shiran Pasternak, Dan K. Forrest, Roger Wise, Doreen Ware, Rod A Wing, Michael S. Waterman, Miron Livny, David C. Schwartz

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

About 85% of the maize genome consists of highly repetitive sequences that are interspersed by low-copy, gene-coding sequences. The maize community has dealt with this genomic complexity by the construction of an integrated genetic and physical map (iMap), but this resource alone was not sufficient for ensuring the quality of the current sequence build. For this purpose, we constructed a genome-wide, high-resolution optical map of the maize inbred line B73 genome containing >91,000 restriction sites (averaging 1 site/∼23 kb) accrued from mapping genomic DNA molecules. Our optical map comprises 66 contigs, averaging 31.88 Mb in size and spanning 91.5% (2,103.93 Mb/∼2,300 Mb) of the maize genome. A new algorithm was created that considered both optical map and unfinished BAC sequence data for placing 60/66 (2,032.42 Mb) optical map contigs onto the maize iMap. The alignment of optical maps against numerous data sources yielded comprehensive results that proved revealing and productive. For example, gaps were uncovered and characterized within the iMap, the FPC (fingerprinted contigs) map, and the chromosome-wide pseudomolecules. Such alignments also suggested amended placements of FPC contigs on the maize genetic map and proactively guided the assembly of chromosome-wide pseudomolecules, especially within complex genomic regions. Lastly, we think that the full integration of B73 optical maps with the maize iMap would greatly facilitate maize sequence finishing efforts that would make it a valuable reference for comparative studies among cereals, or other maize inbred lines and cultivars.

Original languageEnglish (US)
Article numbere1000711
JournalPLoS Genetics
Volume5
Issue number11
DOIs
StatePublished - Nov 2009

Fingerprint

Zea mays
genome
Fluspirilene
maize
Genome
corn
genomics
inbred lines
chromosome
Chromosomes
Interspersed Repetitive Sequences
chromosomes
repetitive sequences
physical chromosome mapping
Information Storage and Retrieval
cereal
cultivar
finishing
comparative study
DNA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Zhou, S., Wei, F., Nguyen, J., Bechner, M., Potamousis, K., Goldstein, S., ... Schwartz, D. C. (2009). A single molecule scaffold for the maize genome. PLoS Genetics, 5(11), [e1000711]. https://doi.org/10.1371/journal.pgen.1000711

A single molecule scaffold for the maize genome. / Zhou, Shiguo; Wei, Fusheng; Nguyen, John; Bechner, Mike; Potamousis, Konstantinos; Goldstein, Steve; Pape, Louise; Mehan, Michael R.; Churas, Chris; Pasternak, Shiran; Forrest, Dan K.; Wise, Roger; Ware, Doreen; Wing, Rod A; Waterman, Michael S.; Livny, Miron; Schwartz, David C.

In: PLoS Genetics, Vol. 5, No. 11, e1000711, 11.2009.

Research output: Contribution to journalArticle

Zhou, S, Wei, F, Nguyen, J, Bechner, M, Potamousis, K, Goldstein, S, Pape, L, Mehan, MR, Churas, C, Pasternak, S, Forrest, DK, Wise, R, Ware, D, Wing, RA, Waterman, MS, Livny, M & Schwartz, DC 2009, 'A single molecule scaffold for the maize genome', PLoS Genetics, vol. 5, no. 11, e1000711. https://doi.org/10.1371/journal.pgen.1000711
Zhou S, Wei F, Nguyen J, Bechner M, Potamousis K, Goldstein S et al. A single molecule scaffold for the maize genome. PLoS Genetics. 2009 Nov;5(11). e1000711. https://doi.org/10.1371/journal.pgen.1000711
Zhou, Shiguo ; Wei, Fusheng ; Nguyen, John ; Bechner, Mike ; Potamousis, Konstantinos ; Goldstein, Steve ; Pape, Louise ; Mehan, Michael R. ; Churas, Chris ; Pasternak, Shiran ; Forrest, Dan K. ; Wise, Roger ; Ware, Doreen ; Wing, Rod A ; Waterman, Michael S. ; Livny, Miron ; Schwartz, David C. / A single molecule scaffold for the maize genome. In: PLoS Genetics. 2009 ; Vol. 5, No. 11.
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