A single peptide-MHC complex positively selects a diverse and specific CD8 T cell repertoire

Baomei Wang, Tina M. Primeau, Nancy Myers, Henry W. Rohrs, Michael L. Gross, Lonnie Lybarger, Ted H. Hansen, Janet M. Connolly

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Pathogen recognition by T cells is dependent on their exquisite specificity for self-major histocompatibility complex (MHC) molecules presenting a bound peptide. Although this specificity results from positive and negative selection of developing T cells in the thymus, the relative contribution of these two processes remains controversial To address the relation between the selecting peptide-MHC complex and the specificity of mature T cells, we generated transgenic mice that express a single peptide-MHC class I complex. We demonstrate that positive selection of CD8 T cells in these mice results in an MHC-specific repertoire. Although selection on a single complex is peptide promiscuous, mature T cells are highly peptide specific. Thus, positive selection imparts MHC and peptide specificity on the peripheral CD8 T cell repertoire.

Original languageEnglish (US)
Pages (from-to)871-874
Number of pages4
JournalScience
Volume326
Issue number5954
DOIs
StatePublished - Nov 6 2009

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Major Histocompatibility Complex
T-Lymphocytes
Peptides
T-Cell Antigen Receptor Specificity
Thymus Gland
Transgenic Mice

ASJC Scopus subject areas

  • General

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Wang, B., Primeau, T. M., Myers, N., Rohrs, H. W., Gross, M. L., Lybarger, L., ... Connolly, J. M. (2009). A single peptide-MHC complex positively selects a diverse and specific CD8 T cell repertoire. Science, 326(5954), 871-874. https://doi.org/10.1126/science.1177627

A single peptide-MHC complex positively selects a diverse and specific CD8 T cell repertoire. / Wang, Baomei; Primeau, Tina M.; Myers, Nancy; Rohrs, Henry W.; Gross, Michael L.; Lybarger, Lonnie; Hansen, Ted H.; Connolly, Janet M.

In: Science, Vol. 326, No. 5954, 06.11.2009, p. 871-874.

Research output: Contribution to journalArticle

Wang, B, Primeau, TM, Myers, N, Rohrs, HW, Gross, ML, Lybarger, L, Hansen, TH & Connolly, JM 2009, 'A single peptide-MHC complex positively selects a diverse and specific CD8 T cell repertoire', Science, vol. 326, no. 5954, pp. 871-874. https://doi.org/10.1126/science.1177627
Wang, Baomei ; Primeau, Tina M. ; Myers, Nancy ; Rohrs, Henry W. ; Gross, Michael L. ; Lybarger, Lonnie ; Hansen, Ted H. ; Connolly, Janet M. / A single peptide-MHC complex positively selects a diverse and specific CD8 T cell repertoire. In: Science. 2009 ; Vol. 326, No. 5954. pp. 871-874.
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