A solid-phase receptor binding assay for 125I-hCG.

M. Bortolussi, Ornella Selmin, A. Colombatti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A solid-phase radioligand-receptor assay (RRA) to measure the binding of 125I-labelled human chorionic gonadotropin (125I-hCG) to target cell membranes has been developed. The binding of 125I-hCG to membranes immobilized on the wells of microtitration plates reached a maximum at about 3 hours at 37 degrees C, was saturable, displayed a high affinity (Ka = 2.4 X 10(9) M-1) and was specifically inhibited by unlabelled hCG. In comparison with RRAs carried out with membranes in suspension, the solid-phase RRA is significantly simpler and much faster to perform as it avoids centrifugation or filtration procedures. The solid-phase RRA was adapted profitably to process large numbers of samples at the same time. It proved particularly useful as a screening assay to detect anti-hCG monoclonal antibodies with high inhibitory activity for binding of 125I-hCG to its receptors.

Original languageEnglish (US)
Pages (from-to)219-235
Number of pages17
JournalJournal of Immunoassay
Volume8
Issue number2-3
StatePublished - 1987
Externally publishedYes

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Radioligand Assay
Chorionic Gonadotropin
Assays
Membranes
Centrifugation
Suspensions
Cell membranes
Monoclonal Antibodies
Cell Membrane
Screening

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Bortolussi, M., Selmin, O., & Colombatti, A. (1987). A solid-phase receptor binding assay for 125I-hCG. Journal of Immunoassay, 8(2-3), 219-235.

A solid-phase receptor binding assay for 125I-hCG. / Bortolussi, M.; Selmin, Ornella; Colombatti, A.

In: Journal of Immunoassay, Vol. 8, No. 2-3, 1987, p. 219-235.

Research output: Contribution to journalArticle

Bortolussi, M, Selmin, O & Colombatti, A 1987, 'A solid-phase receptor binding assay for 125I-hCG.', Journal of Immunoassay, vol. 8, no. 2-3, pp. 219-235.
Bortolussi, M. ; Selmin, Ornella ; Colombatti, A. / A solid-phase receptor binding assay for 125I-hCG. In: Journal of Immunoassay. 1987 ; Vol. 8, No. 2-3. pp. 219-235.
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