A study on proliferation and phenotypical stability of schwann cell on Keratin/PLGA film

A. Young Oh, Soon Hee Kim, Yun Tae Kim, Na Ri Jeon, Jae Chan Yang, Sang Jin Lee, James J. Yoo, Mark Van Dyke, Hyung Sik Shin, John M. Rhee, Gilson Khang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.

Original languageEnglish (US)
Pages (from-to)118-123
Number of pages6
JournalPolymer (Korea)
Volume33
Issue number2
StatePublished - Mar 2009
Externally publishedYes

Keywords

  • Keratin
  • Phenotype
  • PLGA
  • Proliferation
  • Schwann cell

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry

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