A Successful in vitro Propagation Technique for Resurrection Plants of the Selaginellaceae

Rongpei Yu, Anthony E. Baniaga, Stacy A. Jorgensen, Michael S. Barker

Research output: Research - peer-reviewArticle

Abstract

Resurrection plants of the Selaginellaceae are renowned for their ability to tolerate desiccation as well as the small size of their nuclear genomes. These traits position Selaginella as a promising model system to understand many aspects of plant evolution. However, there is not an established method for the laboratory cultivation of resurrection species of Selaginella. We explored methods of in vitro propagation for resurrection species of Selaginella and identified a set of successful techniques. Our in vitro propagation system included two main steps: surface-sterilized megaspores were cultured alone on C-Fern agar medium for three weeks, followed by the addition of surface-sterilized microspores to the germinated megaspore cultures for co-culture. Sporelings of Selaginella eremophila and S. rupincola were observed after 2-5 weeks of co-culture, and all sporelings survived. Our methods aim to further the interest and use of resurrection species of Selaginella for manipulative studies to better understand the biology of desiccation tolerance and their unique genome architecture.

LanguageEnglish (US)
Pages96-104
Number of pages9
JournalAmerican Fern Journal
Volume107
Issue number2
DOIs
StatePublished - Apr 1 2017

Fingerprint

Selaginellaceae
Selaginella
methodology
method
desiccation
genome
megaspores
coculture
fern
agar
tolerance
laboratory
microspores
ferns and fern allies
nuclear genome
laboratory techniques
Biological Sciences

Keywords

  • fertilization
  • megagametophytes
  • microgametophytes
  • Selaginella
  • sporelings

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

A Successful in vitro Propagation Technique for Resurrection Plants of the Selaginellaceae. / Yu, Rongpei; Baniaga, Anthony E.; Jorgensen, Stacy A.; Barker, Michael S.

In: American Fern Journal, Vol. 107, No. 2, 01.04.2017, p. 96-104.

Research output: Research - peer-reviewArticle

Yu, Rongpei ; Baniaga, Anthony E. ; Jorgensen, Stacy A. ; Barker, Michael S./ A Successful in vitro Propagation Technique for Resurrection Plants of the Selaginellaceae. In: American Fern Journal. 2017 ; Vol. 107, No. 2. pp. 96-104
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