A teleost skin bioassay for melanotropic peptides

Ana Maria de L Castrucci, Mac E. Hadley, Victor J Hruby

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A teleost (the eel, Synbranchus marmoratus) skin bioassay for melanotropic peptides and other agonists is described. Unlike previous teleost assays that generally monitor or observe individual melanophores, this objective assay utilizes large intact, pieces-of-skin and quantitative photoreflectance methods. Since melanosomes within most teleost melanophores are generally dispersed, the present assay provides a method for measuring the response of integumental melanophores to melanosome-aggregating agents such as MCH, a putative melanin-concentrating hormone. This bioassay is sensitive to MCH at a concentration as low as 10-12M. Because of the magnitude of this lightening response, four-point dose-response curves can be obtained. Skins lightened by MCH can then be used for bioassay of melanotropins or other melanosome-dispersing agents, such as β-adrenoceptor agonists. This bioassay is unique in providing a method for determining the biological activities of melanotropic peptides with opposing actions.

Original languageEnglish (US)
Pages (from-to)374-380
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume66
Issue number3
DOIs
StatePublished - 1987

Fingerprint

Melanophores
Melanosomes
skin (animal)
Biological Assay
bioassays
peptides
Skin
Peptides
agonists
assays
melanocyte-stimulating hormone
Melanocyte-Stimulating Hormones
Eels
adrenergic receptors
melanin
concentrating
eel
Adrenergic Receptors
dose response
quantitative analysis

ASJC Scopus subject areas

  • Endocrinology

Cite this

A teleost skin bioassay for melanotropic peptides. / Castrucci, Ana Maria de L; Hadley, Mac E.; Hruby, Victor J.

In: General and Comparative Endocrinology, Vol. 66, No. 3, 1987, p. 374-380.

Research output: Contribution to journalArticle

Castrucci, Ana Maria de L ; Hadley, Mac E. ; Hruby, Victor J. / A teleost skin bioassay for melanotropic peptides. In: General and Comparative Endocrinology. 1987 ; Vol. 66, No. 3. pp. 374-380.
@article{ef37a46033a14461b6f192fed3e692df,
title = "A teleost skin bioassay for melanotropic peptides",
abstract = "A teleost (the eel, Synbranchus marmoratus) skin bioassay for melanotropic peptides and other agonists is described. Unlike previous teleost assays that generally monitor or observe individual melanophores, this objective assay utilizes large intact, pieces-of-skin and quantitative photoreflectance methods. Since melanosomes within most teleost melanophores are generally dispersed, the present assay provides a method for measuring the response of integumental melanophores to melanosome-aggregating agents such as MCH, a putative melanin-concentrating hormone. This bioassay is sensitive to MCH at a concentration as low as 10-12M. Because of the magnitude of this lightening response, four-point dose-response curves can be obtained. Skins lightened by MCH can then be used for bioassay of melanotropins or other melanosome-dispersing agents, such as β-adrenoceptor agonists. This bioassay is unique in providing a method for determining the biological activities of melanotropic peptides with opposing actions.",
author = "Castrucci, {Ana Maria de L} and Hadley, {Mac E.} and Hruby, {Victor J}",
year = "1987",
doi = "10.1016/0016-6480(87)90247-4",
language = "English (US)",
volume = "66",
pages = "374--380",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A teleost skin bioassay for melanotropic peptides

AU - Castrucci, Ana Maria de L

AU - Hadley, Mac E.

AU - Hruby, Victor J

PY - 1987

Y1 - 1987

N2 - A teleost (the eel, Synbranchus marmoratus) skin bioassay for melanotropic peptides and other agonists is described. Unlike previous teleost assays that generally monitor or observe individual melanophores, this objective assay utilizes large intact, pieces-of-skin and quantitative photoreflectance methods. Since melanosomes within most teleost melanophores are generally dispersed, the present assay provides a method for measuring the response of integumental melanophores to melanosome-aggregating agents such as MCH, a putative melanin-concentrating hormone. This bioassay is sensitive to MCH at a concentration as low as 10-12M. Because of the magnitude of this lightening response, four-point dose-response curves can be obtained. Skins lightened by MCH can then be used for bioassay of melanotropins or other melanosome-dispersing agents, such as β-adrenoceptor agonists. This bioassay is unique in providing a method for determining the biological activities of melanotropic peptides with opposing actions.

AB - A teleost (the eel, Synbranchus marmoratus) skin bioassay for melanotropic peptides and other agonists is described. Unlike previous teleost assays that generally monitor or observe individual melanophores, this objective assay utilizes large intact, pieces-of-skin and quantitative photoreflectance methods. Since melanosomes within most teleost melanophores are generally dispersed, the present assay provides a method for measuring the response of integumental melanophores to melanosome-aggregating agents such as MCH, a putative melanin-concentrating hormone. This bioassay is sensitive to MCH at a concentration as low as 10-12M. Because of the magnitude of this lightening response, four-point dose-response curves can be obtained. Skins lightened by MCH can then be used for bioassay of melanotropins or other melanosome-dispersing agents, such as β-adrenoceptor agonists. This bioassay is unique in providing a method for determining the biological activities of melanotropic peptides with opposing actions.

UR - http://www.scopus.com/inward/record.url?scp=0023359328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023359328&partnerID=8YFLogxK

U2 - 10.1016/0016-6480(87)90247-4

DO - 10.1016/0016-6480(87)90247-4

M3 - Article

C2 - 3609709

AN - SCOPUS:0023359328

VL - 66

SP - 374

EP - 380

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 3

ER -