A toxin-binding alkaline phosphatase fragment synergizes Bt toxin Cry1Ac against susceptible and resistant Helicoverpa armigera

Wenbo Chen, Chenxi Liu, Yutao Xiao, Dandan Zhang, Yongdong Zhang, Xianchun Li, Bruce E Tabashnik, Kongming Wu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Evolution of resistance by insects threatens the continued success of pest control using insecticidal crystal (Cry) proteins from the bacterium Bacillus thuringiensis (Bt) in sprays and transgenic plants. In this study, laboratory selection with Cry1Ac yielded five strains of cotton bollworm, Helicoverpa armigera, with resistance ratios at the median lethal concentration (LC<inf>50</inf>) of activated Cry1Ac ranging from 22 to 1700. Reduced activity and reduced transcription of an alkaline phosphatase protein that binds Cry1Ac was associated with resistance to Cry1Ac in the four most resistant strains. A Cry1Ac-binding fragment of alkaline phosphatase from H. armigera (HaALP1f) was not toxic by itself, but it increased mortality caused by Cry1Ac in a susceptible strain and in all five resistant strains. Although synergism of Bt toxins against susceptible insects by toxin-binding fragments of cadherin and aminopeptidase N has been reported previously, the results here provide the first evidence of synergism of a Bt toxin by a toxin-binding fragment of alkaline phosphatase. The results here also provide the first evidence of synergism of a Bt toxin by any toxin-binding peptide against resistant insects.

Original languageEnglish (US)
Article numbere0126288
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 17 2015

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Bacillus thuringiensis
Helicoverpa armigera
Bacilli
Alkaline Phosphatase
alkaline phosphatase
Insects
toxins
synergism
CD13 Antigens
Pest Control
Poisons
Genetically Modified Plants
Pest control
Cadherins
insects
Transcription
Cotton
membrane alanyl aminopeptidase
Bacteria
crystal proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A toxin-binding alkaline phosphatase fragment synergizes Bt toxin Cry1Ac against susceptible and resistant Helicoverpa armigera. / Chen, Wenbo; Liu, Chenxi; Xiao, Yutao; Zhang, Dandan; Zhang, Yongdong; Li, Xianchun; Tabashnik, Bruce E; Wu, Kongming.

In: PLoS One, Vol. 10, No. 4, e0126288, 17.04.2015.

Research output: Contribution to journalArticle

Chen, Wenbo ; Liu, Chenxi ; Xiao, Yutao ; Zhang, Dandan ; Zhang, Yongdong ; Li, Xianchun ; Tabashnik, Bruce E ; Wu, Kongming. / A toxin-binding alkaline phosphatase fragment synergizes Bt toxin Cry1Ac against susceptible and resistant Helicoverpa armigera. In: PLoS One. 2015 ; Vol. 10, No. 4.
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