A transmembrane domain and GxxxG motifs within L2 are essential for papillomavirus infection

Matthew P. Bronnimann, Janice A. Chapman, Chad K. Park, Samuel K Campos

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

During cellular invasion, human papillomavirus type 16 (HPV16) must transfer its viral genome (vDNA) across the endosomal membrane prior to its accumulation at nuclear PML bodies for the establishment of infection. After cellular uptake, the capsid likely undergoes pH-dependent disassembly within the endo-/lysosomal compartment, thereby exposing hidden domains in L2 that facilitate membrane penetration of L2/vDNA complexes. In an effort to identify regions of L2 that might physically interact with membranes, we have subjected the L2 sequence to multiple transmembrane (TM) domain prediction algorithms. Here, we describe a conserved TM domain within L2 (residues 45 to 67) and investigate its role in HPV16 infection. In vitro, the predicted TM domain adopts an alpha-helical structure in lipid environments and can function as a real TM domain, although not as efficiently as the bona fide TM domain of PDGFR. An L2 double point mutant renders the TM domain nonfunctional and blocks HPV16 infection by preventing endosomal translocation of vDNA. The TM domain contains three highly conserved GxxxG motifs. These motifs can facilitate homotypic and heterotypic interactions between TM helices, activities that may be important for vDNA translocation. Disruption of some of these GxxxG motifs resulted in noninfectious viruses, indicating a critical role in infection. Using a ToxR-based homo-oligomerization assay, we show a propensity for this TM domain to self-associate in a GxxxG-dependent manner. These data suggest an important role for the self-associating L2 TM domain and the conserved GxxxG motifs in the transfer of vDNA across the endo/lysosomal membrane.

Original languageEnglish (US)
Pages (from-to)464-473
Number of pages10
JournalJournal of Virology
Volume87
Issue number1
DOIs
StatePublished - Jan 2013

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Papillomavirus Infections
Papillomaviridae
Human papillomavirus 16
Membranes
infection
capsid
Viral Genome
Capsid
Homo
Infection
Viruses
uptake mechanisms
Lipids
viruses
mutants
genome
prediction
assays
lipids

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A transmembrane domain and GxxxG motifs within L2 are essential for papillomavirus infection. / Bronnimann, Matthew P.; Chapman, Janice A.; Park, Chad K.; Campos, Samuel K.

In: Journal of Virology, Vol. 87, No. 1, 01.2013, p. 464-473.

Research output: Contribution to journalArticle

Bronnimann, Matthew P. ; Chapman, Janice A. ; Park, Chad K. ; Campos, Samuel K. / A transmembrane domain and GxxxG motifs within L2 are essential for papillomavirus infection. In: Journal of Virology. 2013 ; Vol. 87, No. 1. pp. 464-473.
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