A tuneable array of unique steady-state microfluidic gradients

Matthew D. Estes, Cedric Hurth, Matthew Barrett, Frederic Zenhausern

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report an on-chip gradient generator that has been designed, modelled, fabricated, and characterized to facilitate temporal tuning of several unique gradients in parallel for multiple applications. This design allows for steady state programming of the intensities across multiple orders of magnitude while producing exponential, linear, and logarithmic gradient profiles. The magnitude of the gradients is controlled through regulating the ratio of the two on-chip flow inlets without the need for valves or other active mixers. On-chip binding of biotin by a fluorescent streptavidin complex creates a diffusive barrier that regulates access to the gradient inlets, providing a second orthogonal mechanism for regulating the microgradient intensities. The device is also characterized using an on-chip enzymatic reaction to produce an array of tuneable product concentrations within the various microchannels.

Original languageEnglish (US)
Pages (from-to)12805-12814
Number of pages10
JournalPhysical Chemistry Chemical Physics
Volume15
Issue number31
DOIs
StatePublished - Aug 21 2013

Fingerprint

Inlet flow
Streptavidin
Biotin
Microchannels
Microfluidics
Tuning
gradients
chips
inlet flow
biotin
microchannels
programming
generators
tuning
products
profiles

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

Cite this

A tuneable array of unique steady-state microfluidic gradients. / Estes, Matthew D.; Hurth, Cedric; Barrett, Matthew; Zenhausern, Frederic.

In: Physical Chemistry Chemical Physics, Vol. 15, No. 31, 21.08.2013, p. 12805-12814.

Research output: Contribution to journalArticle

Estes, Matthew D. ; Hurth, Cedric ; Barrett, Matthew ; Zenhausern, Frederic. / A tuneable array of unique steady-state microfluidic gradients. In: Physical Chemistry Chemical Physics. 2013 ; Vol. 15, No. 31. pp. 12805-12814.
@article{3b2abf11685f4ad099131152aeb19ae5,
title = "A tuneable array of unique steady-state microfluidic gradients",
abstract = "We report an on-chip gradient generator that has been designed, modelled, fabricated, and characterized to facilitate temporal tuning of several unique gradients in parallel for multiple applications. This design allows for steady state programming of the intensities across multiple orders of magnitude while producing exponential, linear, and logarithmic gradient profiles. The magnitude of the gradients is controlled through regulating the ratio of the two on-chip flow inlets without the need for valves or other active mixers. On-chip binding of biotin by a fluorescent streptavidin complex creates a diffusive barrier that regulates access to the gradient inlets, providing a second orthogonal mechanism for regulating the microgradient intensities. The device is also characterized using an on-chip enzymatic reaction to produce an array of tuneable product concentrations within the various microchannels.",
author = "Estes, {Matthew D.} and Cedric Hurth and Matthew Barrett and Frederic Zenhausern",
year = "2013",
month = "8",
day = "21",
doi = "10.1039/c3cp44640a",
language = "English (US)",
volume = "15",
pages = "12805--12814",
journal = "Physical Chemistry Chemical Physics",
issn = "1463-9076",
publisher = "Royal Society of Chemistry",
number = "31",

}

TY - JOUR

T1 - A tuneable array of unique steady-state microfluidic gradients

AU - Estes, Matthew D.

AU - Hurth, Cedric

AU - Barrett, Matthew

AU - Zenhausern, Frederic

PY - 2013/8/21

Y1 - 2013/8/21

N2 - We report an on-chip gradient generator that has been designed, modelled, fabricated, and characterized to facilitate temporal tuning of several unique gradients in parallel for multiple applications. This design allows for steady state programming of the intensities across multiple orders of magnitude while producing exponential, linear, and logarithmic gradient profiles. The magnitude of the gradients is controlled through regulating the ratio of the two on-chip flow inlets without the need for valves or other active mixers. On-chip binding of biotin by a fluorescent streptavidin complex creates a diffusive barrier that regulates access to the gradient inlets, providing a second orthogonal mechanism for regulating the microgradient intensities. The device is also characterized using an on-chip enzymatic reaction to produce an array of tuneable product concentrations within the various microchannels.

AB - We report an on-chip gradient generator that has been designed, modelled, fabricated, and characterized to facilitate temporal tuning of several unique gradients in parallel for multiple applications. This design allows for steady state programming of the intensities across multiple orders of magnitude while producing exponential, linear, and logarithmic gradient profiles. The magnitude of the gradients is controlled through regulating the ratio of the two on-chip flow inlets without the need for valves or other active mixers. On-chip binding of biotin by a fluorescent streptavidin complex creates a diffusive barrier that regulates access to the gradient inlets, providing a second orthogonal mechanism for regulating the microgradient intensities. The device is also characterized using an on-chip enzymatic reaction to produce an array of tuneable product concentrations within the various microchannels.

UR - http://www.scopus.com/inward/record.url?scp=84880526571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880526571&partnerID=8YFLogxK

U2 - 10.1039/c3cp44640a

DO - 10.1039/c3cp44640a

M3 - Article

VL - 15

SP - 12805

EP - 12814

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

SN - 1463-9076

IS - 31

ER -