A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Jennifer L. Furman, Pui Wing Mok, Shengyi Shen, Cliff I. Stains, Indraneel Ghosh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

Original languageEnglish (US)
Pages (from-to)397-399
Number of pages3
JournalChemical Communications
Volume47
Issue number1
DOIs
StatePublished - Jan 7 2011

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Poly Adenosine Diphosphate Ribose
Firefly Luciferases
Administrative data processing
Cell death
Luciferases
Adenosine Diphosphate
DNA
Repair
Cells
Monitoring
Sensors

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death. / Furman, Jennifer L.; Mok, Pui Wing; Shen, Shengyi; Stains, Cliff I.; Ghosh, Indraneel.

In: Chemical Communications, Vol. 47, No. 1, 07.01.2011, p. 397-399.

Research output: Contribution to journalArticle

Furman, Jennifer L. ; Mok, Pui Wing ; Shen, Shengyi ; Stains, Cliff I. ; Ghosh, Indraneel. / A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death. In: Chemical Communications. 2011 ; Vol. 47, No. 1. pp. 397-399.
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