A UAV-Mounted Whole Cell Biosensor System for Environmental Monitoring Applications

Yi Lu, Dominique Macias, Zachary S. Dean, Nicole R. Kreger, Pak Kin Wong

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This study reports the development of a portable whole cell biosensor system for environmental monitoring applications, such as air quality control, water pollution monitoring, and radiation leakage detection. The system consists of a lightweight mechanical housing, a temperature regulating system, and a microfluidic bacterial inoculation channel. The overall system, which is less than 200 g, serves as a portable incubator for cell inoculation and can be mounted on an unmanned aerial vehicle for monitoring remote and unreachable locations. The feedback control system maintains the inoculation temperature within 0.05 °C. The large surface-to-volume ratio of the polydimethylsiloxane microchannel facilitates effective gas exchange for rapid bacterial growth. Molecular dynamic simulation shows effective diffusion of major gas pollutants in PDMS toward gas sensing applications. By optimizing the design, we demonstrate the operation of the system in ambient temperatures from 5 °C to 32 °C and rapid bacterial growth in microchannels compared to standard bacterial culture techniques.

Original languageEnglish (US)
Article number7328305
Pages (from-to)811-817
Number of pages7
JournalIEEE Transactions on Nanobioscience
Volume14
Issue number8
DOIs
StatePublished - Dec 1 2015

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Environmental Monitoring
Biosensing Techniques
Unmanned aerial vehicles (UAV)
Biosensors
Gases
Microchannels
Temperature
Monitoring
Radiation Monitoring
Water Pollution
Incubators
Culture Techniques
Water pollution
Microfluidics
Polydimethylsiloxane
Molecular Dynamics Simulation
Growth
Air quality
Quality Control
Feedback control

Keywords

  • Environmental monitoring
  • gas diffusivity
  • microfluidics
  • PDMS
  • PID control
  • portable incubator
  • whole-cell biosensor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Biomedical Engineering
  • Biotechnology
  • Computer Science Applications
  • Bioengineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

A UAV-Mounted Whole Cell Biosensor System for Environmental Monitoring Applications. / Lu, Yi; Macias, Dominique; Dean, Zachary S.; Kreger, Nicole R.; Wong, Pak Kin.

In: IEEE Transactions on Nanobioscience, Vol. 14, No. 8, 7328305, 01.12.2015, p. 811-817.

Research output: Contribution to journalArticle

Lu, Yi ; Macias, Dominique ; Dean, Zachary S. ; Kreger, Nicole R. ; Wong, Pak Kin. / A UAV-Mounted Whole Cell Biosensor System for Environmental Monitoring Applications. In: IEEE Transactions on Nanobioscience. 2015 ; Vol. 14, No. 8. pp. 811-817.
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