A universal electrode approach for automated electrochemical detection of bacterial 16S rRNA

Mandy L. Y Sin, Vincent Gau, Joseph C. Liao, P. K. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Transforming microfluidics based biosensing systems from laboratory research into clinical reality remains an elusive goal despite decades of intensive research. A fundamental obstacle towards the development of fully automated microfluidic diagnostic systems is the lack of an effective strategy for combining multiple pumping., sample preparation, and detection modules into an integrated platform. Herein., we report a universal electrode approach, which incorporates DC electrolytic pumping, AC electrokinetic sample preparation, and self-assembled monolayer based electrochemical sensing on a single micro fluidic platform, to automate complicated molecular analysis procedures in non-traditional healthcare settings. Using the universal electrode approach, major microfluidic operations required in molecular diagnostics, such as pumping., mixing., washing, and sensing can be performed in a single platform. We demonstrate the universal electrode platform for detecting bacterial 16S rRNA, a phylogenetic marker., toward rapid diagnostics of urinary tract infection. Since only electronic interfaces are required to operate the platform, the universal electrode approach represents an effective system integration strategy to realize the potential of micro fluidics in molecular diagnostics at the point of care.

Original languageEnglish (US)
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages96-100
Number of pages5
DOIs
StatePublished - 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: Nov 4 2012Nov 7 2012

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period11/4/1211/7/12

Keywords

  • 16s rRNA
  • Electrochemical detection
  • Electrokinetics
  • Lab-on-a-chip
  • Microfluidic integration

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

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  • Cite this

    Y Sin, M. L., Gau, V., Liao, J. C., & Wong, P. K. (2012). A universal electrode approach for automated electrochemical detection of bacterial 16S rRNA. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 96-100). [6509117] (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509117