A universal electrode approach for automated electrochemical molecular analyses

Mandy Lai Yi Sin, Vincent Gau, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Transforming microfluidics-based biosensing systems from laboratory research into clinical reality remains an elusive goal despite decades of intensive research. A fundamental obstacle for the development of fully automated microfluidic diagnostic systems is the lack of an effective strategy for combining pumping, sample preparation, and detection modules into an integrated biosensing platform. Herein, we report a universal electrode approach, which incorporates dc electrolytic pumping, ac electrokinetic sample preparation, and self-assembled monolayer based electrochemical sensing on a single microfluidic platform, to automate complicated molecular analysis procedures that will enable biosensing applications in nontraditional health care settings. Using the universal electrode approach, major microfluidic operations required in molecular analyses, such as pumping, mixing, washing, and sensing, can be performed in a single platform. We demonstrate the universal electrode platform for detecting bacterial 16S rRNA, a phylogenetic marker, toward rapid diagnostics of urinary tract infection. Since only electronic interfaces are required to operate the platform, the universal electrode approach represents an effective system integration strategy to realize the potential of microfluidics in molecular diagnostics at the point of care. \hfill{[2012\hbox{-}0310]}

Original languageEnglish (US)
Article number6506946
Pages (from-to)1126-1132
Number of pages7
JournalJournal of Microelectromechanical Systems
Volume22
Issue number5
DOIs
StatePublished - Oct 14 2013

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Keywords

  • 16S rRNA
  • Electrochemical detection
  • Electrokinetics
  • System integration
  • Urinary tract infection

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering

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